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Early gene expression of regenerating zebrafish hearts following water or atropine treatment

ABSTRACT: We report global RNA expression profiles from whole zebrafish hearts 24 hours after ventricle amputation. Zebrafish were exposed to atropine or water following surgery.

ORGANISM(S): Danio rerio

PROVIDER: GSE69775 | GEO | 2015/06/11

SECONDARY ACCESSION(S): PRJNA286744

REPOSITORIES: GEO

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Early gene expression of regenerating zebrafish hearts following water or atropine treatment

Project description:We report global RNA expression profiles from whole zebrafish hearts 24 hours after ventricle amputation. Zebrafish were exposed to atropine or water following surgery. 15 zebrafish hearts were pooled per microarray chip. Amputated hearts of zebrafish exposed to atropine was compared to hearts of zebrafish exposed to water.

2015-06-11 | E-GEOD-69775 | biostudies-arrayexpress

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Project description:Adult zebrafish hearts have the ability to regenerate. The roles of non-myocytes in this process have remained elusive. Here, we have performed 2 scRNAseq experiments on interstitial cells. Experiment 1 (E1) included interstitial cells obtained from uninjured, regenerating (3 days, 7 days and 14 days post-apical amputation). Experiment 2 (E2) included cells from uninjured, sham-operated (abdomen opened) and regenerating (3 days post-amputation) with and without MMP inhibitor (NSC40520) treatment. Cells were obtained by heart dissection followed by enzymatic dissociation and FACS sorting of single, viable nucleated cells.

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Project description:In contrast to mammals, the adult zebrafish hearts can regenerate within a short period. Upregulation of major vault protein (Mvp) expression after ventricle resection. To determine the impact of loss of mvp on transcriptome during heart regeneration, we pursued transcriptome analysis (RNA-Seq) on wild type and mvp mutant zebrafish hearts at 7 day-post-amputation.

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