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MTORC inhibition of neuroblastoma SK-N-BE(2)c cells cultured under hypoxic conditions

ABSTRACT: Differential expression of the oxygen sensitive hypoxia-inducible transcription factor (HIF) subunits HIF-1a and HIF-2a occurs in many tumor types, but the underlying regulatory mechanisms remain poorly understood. Here we investigate the role of mTOR complex in the regulation of HIF expression in cells cultured under hypoxic conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE69833 | GEO | 2016/01/01

SECONDARY ACCESSION(S): PRJNA286890

REPOSITORIES: GEO

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mTORC inhibition of neuroblastoma SK-N-BE(2)c cells cultured under hypoxic conditions

Project description:Differential expression of the oxygen sensitive hypoxia-inducible transcription factor (HIF) subunits HIF-1a and HIF-2a occurs in many tumor types, but the underlying regulatory mechanisms remain poorly understood. Here we investigate the role of mTOR complex in the regulation of HIF expression in cells cultured under hypoxic conditions. Neuroblastoma SK-N-BE(2)c cells were treated with DMSO or the mTORC complex inhibitor PP242 and cultured at hypoxia for 24, 48 or 72 hours.

2016-07-03 | E-GEOD-69833 | biostudies-arrayexpress

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