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Triple Inhibition of TGFb/BMP/GSK3 Signaling in Human Pluripotent Stem Cells Generates PAX6- Neural Progenitors with robust Self-renewal

ABSTRACT: In this report, we show that in addition to dual SMAD inhibition, blocking Glycogen synthase kinase 3(GSK3) signaling by adding CHIR99021 generated novel NPCs with significantly enhanced proliferation. By RNA-seq, we show that S/D/C NPCs and S/D NPCs display distinct expression profiles, as S/D/C triggered NPCs are Pax6- and express hindbrain genes while . S/D triggered NPCs are Pax6+ and highly express forebrain genes. Our data reveal that Pax6 labels distinct regionally specified hNPCs and manipulating GSK3 signaling could pattern hNPCs with positionally specified fate and different self-renewal capability.

ORGANISM(S): Homo sapiens

PROVIDER: GSE69891 | GEO | 2018/06/15

REPOSITORIES: GEO

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