ChIP-chip analyses of H3K9 acetylation in WT, alp13 deletion and pst1-1 mutant S.pombe cells.
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ABSTRACT: ChIP-chip analyses of H3K9 acetylation in WT, alp13 deletion (Clr6-complex II mutant) and pst1-1 (Clr6-complex I mutant) S.pombe cells. Keywords: ChIP-chip
Project description:Expression analyses in alp13 deletion (Clr6-complex II mutant), clr6-1 partial mutant (Clr6 complexes I and II mutant) and pst1-1 partial mutant (Clr6-complex I mutant) S.pombe cells. Keywords: Expression
Project description:Expression profile of mutant cells compared to wild-type cells: Expression profile of Schizosaccharomyces pombe genome in ∆pht1, ∆ago1, ∆clr4, rik1, ∆pht1rik1, ∆pht1∆ago1 and ∆pht1∆clr4 cells. Expression profile of Schizosaccharomyces pombe genome in ∆rrp6, ∆cid14, ∆swi6, ∆swr1, ∆set1, ∆pht1∆swi6 and ∆pht1∆set1 cells. Expression profile of Schizosaccharomyces pombe genome in ∆alp13, ∆cph1, ∆set2, clr6-1 and ∆cph1∆pht1 cells. Occupancy profiling: Occupancy profiling of RNA polymerase II, histone variant H2A.Z and ClrC subunit Rik1 in fission yeast Schizosaccharomyces pombe Occupancy profiling of histone variant H2A.Z in ∆msc1 cells.
Project description:Histone acetylation plays an important role in regulating DNA accessibility. Multifunctional Sin3 proteins bind histone deacetylases (HDACs) to assemble silencing complexes that selectively target chromatin. We show that, in fission yeast, an essential HDAC Clr6 exists in two distinct Sin3 core complexes. Complex I contains an essential Sin3 homologue Pst1 and other factors, and predominantly targets gene promoters. Complex II contains a non-essential Sin3, Pst2, and several conserved proteins. It preferentially targets transcribed chromosomal regions and centromere cores. Defects in complex II abrogate global protective functions of chromatin, causing increased accessibility of DNA to genotoxic agents and widespread antisense transcripts that are processed by the exosome. Interestingly, the two Clr6 complexes differentially repress forward and reverse centromeric repeat transcripts, suggesting that these complexes regulate transcription in heterochromatin and euchromatin in a similar manner, including suppression of spurious transcripts from cryptic start sites. This SuperSeries is composed of the SubSeries listed below.