TL2 14028s invA::cam IP BALBc mouse LibS invA vs Lib0 invA Mice
Ontology highlight
ABSTRACT: Salmonella typhimurium 14028s invA::cam Transposon library recovered from spleen 2 days after IP passage of 2.2 x 10^6 through BALB/c mouse, compared to the initial Transposon library grown in LB broth (kanamycin 50ug/ml), O/N at 37°C with aeration Keywords: Transposon tag analysis
Project description:Salmonella typhimurium 14028s Transposon library recovered from spleen after IP passage of 2x10^6 through BALB/c mouse, compared to the initial Transposon library grown in LB broth (kanamycin 50ug/ml), O/N at 37°C with aeration Keywords: Transposon tag analysis
Project description:Salmonella typhimurium 14028s Transposon library recovered after three consecutive rounds of growth to late log phase in M9 minimal medium (arabinose 0.4%) at 37°C with aeration, compared to an expansion of the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis
Project description:Salmonella typhimurium 14028s invA::cam Transposon library recovered from spleen 2 days after IP passage of 2.2 x 10^6 through BALB/c mouse, compared to the initial Transposon library grown in LB broth (kanamycin 50ug/ml), O/N at 37°C with aeration Keywords: Transposon tag analysis 6 biological replicates analysed after arbitrarily primed sampling (Klenow, DOPR degenerate primer) + specific PCR amplification (Taq DNA Pol) + T7 in vitro transcription (AmpliScribe T7) + RT labeling (Cy3) + hybridization, compared to identically processed standard library (Cy5)
Project description:Salmonella typhimurium 14028s Transposon library grown in M9 minimal medium (arabinose 0.4%), O/N at 37°C with aeration, compared to the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis
Project description:Salmonella typhimurium 14028s Transposon library recovered from spleen after IP passage of 2x10^6 through BALB/c mouse, compared to the initial Transposon library grown in LB broth (kanamycin 50ug/ml), O/N at 37°C with aeration Keywords: Transposon tag analysis 7 biological replicates analysed after arbitrarily primed sampling (DOPR) + specific PCR amplification + T7 in vitro transcription + RT labeling (Cy3) + hybridization, compared to identically processed standard library (Cy5)
Project description:Salmonella typhimurium 14028s Transposon library grown in M9 minimal medium (arabinose 0.4%), O/N at 37°C with aeration, compared to the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis 3 biological replicates analysed after arbitrarily primed sampling (DOPR) + specific PCR amplification + T7 in vitro transcription + RT labeling (Cy3) + hybridization , compared to identically processed standard library (Cy5)
Project description:Salmonella typhimurium 14028s Transposon library recovered after three consecutive rounds of growth to late log phase in M9 minimal medium (arabinose 0.4%) at 37°C with aeration, compared to an expansion of the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis 2 biological replicates analysed after arbitrarily primed sampling (Klenow, DOPR degenerate primer) + specific PCR amplification (Taq DNA Pol) + T7 in vitro transcription (AmpliScribe T7) + RT labeling (Cy3) + hybridization, compared to identically processed standard library (Cy5)
Project description:Salmonella typhimurium 14028s promoter library (180,000 clones, both stable and destabilized GFP versions) recovered from either spleens / PC3 tumors 2 days after IV / IT delivery into nude mice, sorted for green bacteria using FACS, compared to the initial promoter library grown in LB Ampicillin broth, O/N at 37°C with aeration Keywords: promoter screen