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Gene expression profiles of head and neck squamous cell carcinoma (HNSCC) cell lines

ABSTRACT: Chromosomal comparative genomic hybridization (CGH) and oligonucleotide microarrays were used to examine 8 HNSCC cell lines and a plot of gene expression levels relative to their position on the chromosome was produced. Three highly up-regulated genes, NT5C3, ANLN and INHBA, were identified on chromosome 7p14. These genes were subjected to quantitative real-time RT-PCR on cDNA and genomic DNA derived from 8 HNSCC cell lines. ANLN and INHBA showed a strong positive correlation between mRNA expression and genomic DNA levels and a similar relationship was shown for the known oncogene, EGFR, at 7p11.2. In clinical samples, ANLN and INHBA showed a significantly higher expression in tumors than in normal tissues. Patients with high expression levels of INHBA had a shorter disease-free survival rate. Therefore, INHBA may be a promising prognostic marker of HNSCC. Keywords: Identification of molecular targets based on genome-wide gene expression profiling

ORGANISM(S): Homo sapiens

PROVIDER: GSE7073 | GEO | 2007/03/01

SECONDARY ACCESSION(S): PRJNA98481

REPOSITORIES: GEO

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Gene expression analysis of head and neck squamous cell carcinoma (HNSCC) cell lines.

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