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Differentiation of human embryonic stem cells towards hepatocytes

ABSTRACT: To understand in detail the molecular phases that occur during the differentiation of human embryonic stem cells to hepatocyte-like cells we performed an RNA-seq time course of the stages of differentiation across the 21 day differentiation time course. We used a chemically defined (serum-free) protocol, modified from Si-Tayeb et al., 2010 and Song et al., 2009. We describe acquisition of definitive endoderm characteristics at day 3, followed by hepatoblast charcter at day 7-13 and then maturation to hepatocyte-like cells at day 21.

ORGANISM(S): Homo sapiens

PROVIDER: GSE70741 | GEO | 2017/04/28

SECONDARY ACCESSION(S): PRJNA289470

REPOSITORIES: GEO

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Differentiation of human embryonic stem cells towards hepatocytes

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