Expression data from colon cancer cell HCT116 mouse xenograft - Control vs. traditional Chinese medicine Astragalus membranaceus-treated conditions
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ABSTRACT: To identify biomarkers regulated by traditional Chinese medicine Astragalus membranaceus Fischer Bge. var. mongolicus Bge. Hsiao in colorectal cancer. We have identified several differentially expressed genes including microRNAs using Affymetrix HTA-2.0 array. In this dataset, we include the expression data obtained from colon cancer cell line HCT116 grafted into nude mice. The mice was treated either water or traditional Chinese medicine Astragalus membranaceus for 28 days. These data are used to obtain 1425 genes that are differentially expressed in response to Astragalus membranaceus treatment.
Project description:Peripheral nerve injury (PNI) results in loss of neural control and severe disabilities in patients. Promoting functional nerve recovery by accelerating angiogenesis is a promising neuroprotective treatment strategy. Here, we identified a bioactive Radix Astragalus polysaccharide (RAP) extracted from traditional Chinese medicine (TCM) as a potent enhancer of axonal regeneration and remyelination. Notably, RAP promoted functional recovery and delayed gastrocnemius muscle atrophy in a rat model of sciatic nerve crush injury. Further, RAP treatment induced angiogenesis in vivo. Moreover, our in vitro results showed that the role of RAP in endothelial cell (EC) migration and tube formation was probably related to the activation of AKT/eNOS signaling pathway. Altogether, our results show that RAP can enhance functional recovery by accelerating angiogenesis, thereby providing a polysaccharide-based therapeutic strategy for PNI.
Project description:A traditional Chinese medicine (TCM) formula, containing Astragalus membranaceus (Fisch.) Bunge, Aconitum wilsonii Stapf ex Veitch, Curcuma longa L., and Radix ophiopogonis (AACO), has clinically proven therapeutic value for the treatment of chronic heart failure (CHF). In this study, we explored the potential pharmacological mechanism underlying the activity of the AACO formula against CHF.
Project description:Using high-throughput antibody microarray, through cross-sectional sample detection and verification of samples that had undergone physical changes over time, it was found that people with balanced constitution and dampness constitution in Chinese medicine showed differences in serum protein expression. The differences were expressed as the level changes of 19 proteins such as Dectin-2, Siglec-7, AIF and TACI. The research results provided the basis for the scientific expression of traditional Chinese medicine (TCM) constitution.
Project description:Interventions: Traditional Chinese medicine and Western medicine group:Traditional Chinese medicine and Western medicine;Western medicine group:Western medicine
Primary outcome(s): Overall Survival;Progression-free Survival
Study Design: Cohort study
Project description:This SuperSeries is composed of the following subset Series: GSE32083: Transcriptomics of the traditional Japanese medicine juzentaihoto (JTX) on large intestines on germfree mice GSE32084: Transcriptomics of the traditional Japanese medicine juzentaihoto (JTX) on small intestines on germfree mice Refer to individual Series