Genomics

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Dynamic and flexible bridging of H3K9me3-nucleosomes via HP1β-dimerization establishes a plastic state of condensed chromatin


ABSTRACT: Histone H3 trimethylation of lysine 9 (H3K9me3) and heterochromatin proteins 1 (HP1) are hallmarks of heterochromatin, a state of compacted DNA essential for genome stability and long-term transcriptional silencing. The mechanisms by which H3K9me3 and HP1 contribute to chromatin compaction have been speculative and controversial. We demonstrate that mammalian HP1β is a prototypic HP1 protein exemplifying most basal chromatin binding and effects. These are caused by dimeric and dynamic interaction with highly enriched H3K9me3 and are modulated by various electrostatic interfaces. Via internucleosomal bridging HP1β specifically interacts with condensed chromatin, which we postulate stabilizes the compacted state. In agreement, HP1β genome-wide localization follows enrichment of H3K9me3 and bridging of chromatin fibers in a cellular context is sufficient for maintaining heterochromatic conformation. Overall, our findings define a fundamental mechanism for chromatin higher order structural changes caused by HP1 proteins, which may contribute to the plastic nature of condensed chromatin.

ORGANISM(S): Mus musculus

PROVIDER: GSE71114 | GEO | 2017/02/10

SECONDARY ACCESSION(S): PRJNA290375

REPOSITORIES: GEO

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