Transcriptomics

Dataset Information

0

T cell help controls the speed of the cell cycle in germinal center B cells.


ABSTRACT: The germinal center (GC) is a microanatomical compartment wherein high-affinity antibody-producing B cells are selectively expanded. B cells proliferate and mutate their antibody genes in the dark zone (DZ) of the GC and are then selected by T cells in the light zone (LZ) on the basis of affinity. Here, we show that T cell help regulates the speed of cell cycle phase transitions and DNA replication of GC B cells. Genome sequencing and single-molecule analyses revealed that T cell help shortens S phase by regulating replication fork progression while preserving the relative order of replication origin activation. Thus, high-affinity GC B cells are selected by a mechanism that involves prolonged dwell time in the DZ where selected cells undergo accelerated cell cycles.

ORGANISM(S): Mus musculus

PROVIDER: GSE71295 | GEO | 2015/08/12

SECONDARY ACCESSION(S): PRJNA290858

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-08-12 | E-GEOD-71295 | biostudies-arrayexpress
2020-12-10 | GSE162182 | GEO
2015-11-23 | E-GEOD-68043 | biostudies-arrayexpress
2021-09-22 | GSE184475 | GEO
2015-11-23 | GSE68043 | GEO
2012-09-24 | GSE38304 | GEO
2017-03-21 | GSE93554 | GEO
2012-09-24 | E-GEOD-38304 | biostudies-arrayexpress
2019-11-11 | GSE120728 | GEO
2019-11-11 | GSE120727 | GEO