Transcriptomics

Dataset Information

0

Identification of qkia/c target genes


ABSTRACT: Quaking are RNA binding proteins, which are known to regulate the expression of different genes at the post-transcriptional level. Genetic interference with quaking a (qkia) and quaking c (qkic) leads to major myofibril defects during zebrafish development, without affecting early muscle differentiation. In order to understand how qkia and qkic jointly regulate myofibril formation, we performed a comparative analysis of the transcriptome of qkia/qkic (qkia mutant injected with qkic morpholino) versus control embryos. We show that Quaking activity is required for accumulation of the muscle-specific tropomyosin 3 transcript, tpm3.1. Whereas interference with tmp3.1 function disrupts myofibril formation, reintroducing tpm3.1 transcripts into embryos with reduced Quaking activity can restore structured myofibrils. Thus, we identify tropomyosin as an essential component in the process of myofibril formation and as a relay downstream of the regulator proteins Quaking.

ORGANISM(S): Danio rerio

PROVIDER: GSE71573 | GEO | 2017/09/17

SECONDARY ACCESSION(S): PRJNA291531

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-12-01 | GSE107247 | GEO
2024-03-08 | PXD043308 | Pride
2018-05-15 | GSE109012 | GEO
2011-11-04 | E-GEOD-33353 | biostudies-arrayexpress
2020-10-27 | GSE160098 | GEO
2022-03-24 | GSE199093 | GEO
2011-11-04 | GSE33353 | GEO
| PRJNA515372 | ENA
2024-01-04 | GSE245760 | GEO
2014-12-23 | E-GEOD-63707 | biostudies-arrayexpress