Project description:M. smegmatis grown on cholesterol or glycerol in continous culture at a dilution rate of 0.01 h-1. Two-condition experiment, cholesterol chemostat vs. glycerol chemostat. Biological replicates: 4, two dye swaps
Project description:The aim of the experiment is to study the selective gene induction when the bacteria Mycobacterium smegmatis mc M-2 155 grows in 1.8 mM cholesterol. As a control we used RNA of cultures grown in 18 mM glycerol. In both cases the RNA was extracted when the bacteria was in logarithmic phase of growth. We made 3 biological replicates and each of these biological replicates had a technical replicate (dye swap)
Project description:Transcriptional profiling of M. smegmatis and Dcrp1 grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol. Transcriptional profiling of M.smegmatis HLA102 harboring tetracycline inducible vector pMind containing the Crp2 and M. smegmatis harboring pMind grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol
Project description:Transcriptional profiling of M. smegmatis MB100pruR vs. MB100 (isogenic parent) grown in serumvials on Hartmans de Bont medium supplemented with glycerol and proline
Project description:Transcriptional profiling of M. smegmatis mc2155 grown on Hartmans de Bont (HdB) supplemented with 0.2% glycerol and 0.05% Tween80 challenged with 2 μg/ml Bedaquiline and Dimethyl sulfoxide (DMSO)
Project description:Microarray analyses were performed on Wildtype Mycobacterium smegmatis and MSMEG_1918 gene knockout (ΔpdtaS) grown in two different carbon sources, glucose and glycerol.
Project description:Transcriptional profiling of M. smegmatis JR121 expressing VapC and VapBC grown in flasks on Hartmans de Bont medium supplemented with 0.2% glycerol
Project description:To identify the AmtR regulon of Mycobacterium smegmatis, we created a markerless deletion of the amtR gene in the background of strain M. smegmatis mc2155 (wild-type) and compared the transcription profile of both strains grown in batch culture under aerobic conditions on Hartmans de Bont medium supplemented with glycerol (carbon source) and lysine (sole nitrogen source) using microarray. Cells were harvested in early exponential growth stage.
Project description:Transcriptional profiling of M. smegmatis MB100pruR vs. MB100 (isogenic parent) grown in serumvials on Hartmans de Bont medium supplemented with glycerol and proline Two-condition experiment. Biological replicates: 4 independently grown and harvested. One replicate per array.
Project description:Transcriptional profiling of M. smegmatis and Dcrp1 grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol. Transcriptional profiling of M.smegmatis HLA102 harboring tetracycline inducible vector pMind containing the Crp2 and M. smegmatis harboring pMind grown in flasks on Hartmans de Bont medium supplemented with 10 mM glycerol Comparing transcriptional response of wild-type compared with the deletion of crp1 gene (Msmeg_0539). Biological replicates: 4 independently grown and harvested. One replicate per array. Comparing transcriptional response of strain HLA102 to conditional expression of Crp2 (Msmeg_6189) compared with the wild-type with empty vector pMind. Biological replicates: 4 independently grown and harvested. One replicate per array.