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Activin/Nodal signaling supports retinal progenitor specification in a narrow time window during pluripotent stem cell differentiation to anterior neuroectoderm

ABSTRACT: In this study, we used chemically-defined media to induce the in vitro differentiation of mouse embryonic stem cells (ESCs) towards eye field fates. Inhibition of Wnt/ß-Catenin signalling was sufficient to drive ESCs to telencephalic, but not retinal fates. Instead, retinal progenitors could be generated from competent differentiating mouse and human ESCs by the activation of Activin/Nodal signaling within a narrow temporal window corresponding to the emergence of primitive anterior neural progenitors. Our results reveal insights into the mechanisms of eye field specification and open new avenues towards the generation of retinal progenitors for translational medicine.

ORGANISM(S): Mus musculus

PROVIDER: GSE71830 | GEO | 2015/10/01

SECONDARY ACCESSION(S): PRJNA292163

REPOSITORIES: GEO

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