Genomics

Dataset Information

0

Genomic nucleosome organization reconstituted with pure proteins


ABSTRACT: Chromatin remodelers regulate genes by organizing nucleosomes around promoters, but their individual contributions are obfuscated by the complex in vivo milieu of factor redundancy and indirect effects. Genome-wide reconstitution of promoter nucleosome organization with purified proteins resolves this problem and is therefore a critical goal. Here we reconstitute four stages of nucleosome architecture using purified components: Yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and remodelers RSC, ISW2, INO80, and ISW1a. We identify direct, specific and sufficient contributions that in vivo observations validate. First, RSC clears promoters by translating poly(dA:dT) into directional nucleosome removal. Second, partial redundancy is recapitulated where INO80 alone, or ISW2 at Abf1/Reb1sites, positions +1 nucleosomes. Third, INO80 and ISW2 each align downstream nucleosomal arrays. Fourth, ISW1a tightens the spacing to canonical repeat lengths. Such a minimal set of rules and proteins establishes core mechanisms by which promoter chromatin architecture arises through a blend of redundancy and specialization.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE72106 | GEO | 2016/08/14

SECONDARY ACCESSION(S): PRJNA292928

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-08-14 | E-GEOD-72106 | biostudies-arrayexpress
2020-02-10 | GSE140614 | GEO
2015-04-01 | E-GEOD-65593 | biostudies-arrayexpress
2015-04-01 | E-GEOD-65592 | biostudies-arrayexpress
2015-04-01 | GSE65593 | GEO
2015-04-01 | GSE65592 | GEO
2015-04-01 | GSE65591 | GEO
2015-04-01 | GSE65590 | GEO
2015-04-01 | GSE65589 | GEO
2015-04-01 | E-GEOD-65589 | biostudies-arrayexpress