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Next Generation Sequencing Facilitates Quantitative Analysis of Sensory Neurons Transcriptomes in response to Trophic Withdrawal

ABSTRACT: Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to uncover the changes in the transcriptome of sensory neurons in response to trophic withdrawal

ORGANISM(S): Mus musculus

PROVIDER: GSE72129 | GEO | 2016/11/25

SECONDARY ACCESSION(S): PRJNA293123

REPOSITORIES: GEO

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Ontogeny and Trophic Factor Sensitivity of Gastrointestinal Projecting Vagal Sensory Cell Types

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Next Generation Sequencing Facilitates Quantitative Analysis of GlyTranscriptomes

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived interferon-stimulated MEF cells transcriptome profiling (RNA-seq).

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Next-Generation Sequencing Facilitates Quantitative Analysis of Interferon-Stimulated MEFs Transcriptomes [RNA-Seq]

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Next Generation Sequencing Facilitates Quantitative Analysis of Normal Human Kidney Transcriptomes

Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived normal human kidney transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis The kidney tissue was immediately placed and stored in RNAlater® (Ambion), according to the manufacturer’s instruction. The tissue was manually microdissected under microscope in RNAlater® pool for glomerular and tubular compartment. Dissected tissue was homogenized and RNA was prepared using RNAeasy mini columns (Qiagen, Valencia, CA, US), according to the manufacturer’s instructions. RNA quality and quantity were determined using the Laboratory-on-Chip Total RNA PicoKit Agilent BioAnalyzer. Only samples without evidence of degradation were further used (RNA Integrity Number >6).

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