Transcriptomics

Dataset Information

0

Effect of cis-2-dodecenoic acid on F. novicida gene expression


ABSTRACT: In many bacteria, the ability to modulate biofilm production relies on specific signaling molecules that are either self-produced or made by neighboring microbes within the ecological niche. We analyzed the potential interspecies signaling effect of the Burkholderia diffusible signal factor (BDSF) on Francisella novicida, a model organism for Francisella tularensis, and demon- strated that BDSF both inhibits the formation and causes the dispersion of Francisella biofilm. Specificity was demonstrated for the cis versus the trans form of BDSF. Using transcriptome sequencing, quantitative reverse transcription-PCR, and activity as- says, we found that BDSF altered the expression of many F. novicida genes, including genes involved in biofilm formation, such as chitinases. Using a chitinase inhibitor, the antibiofilm activity of BDSF was also shown to be chitinase dependent. In addition, BDSF caused an increase in RelA expression and increased levels of (p)ppGpp, leading to decreased biofilm production. These results support our observation that exposure of F. novicida to BDSF causes biofilm dispersal. Furthermore, BDSF upregulated the genes involved in iron acquisition (figABCD), increasing siderophore production. Thus, this study provides the first evidence for a potential role and mechanism of diffusible signal factor (DSF) signaling in the genus Francisella and suggests the possibility of interspecies signaling between Francisella and other bacteria. Overall, this study suggests that in response to the interspecies DSF signal, F. novicida can alter its gene expression and regulate its biofilm formation.

ORGANISM(S): Francisella tularensis subsp. novicida U112

PROVIDER: GSE72142 | GEO | 2015/08/19

SECONDARY ACCESSION(S): PRJNA293200

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-11-30 | GSE39647 | GEO
2012-11-30 | E-GEOD-39647 | biostudies-arrayexpress
2011-04-02 | E-GEOD-27809 | biostudies-arrayexpress
2020-05-22 | GSE150985 | GEO
2011-04-02 | GSE27809 | GEO
2007-05-23 | E-MEXP-1105 | biostudies-arrayexpress
2008-08-13 | GSE12108 | GEO
2007-05-23 | E-GEOD-7684 | biostudies-arrayexpress
2017-05-02 | GSE75689 | GEO
2015-01-02 | GSE53255 | GEO