Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling of transmitochondrial cybrids (triple negative breast cancer cells in SUM159 background)

ABSTRACT: We used a transmitochondrial cybrid (cybrids)-based discovery approach to identify mitochondria-regulated cancer pathways in TN BCa. Cybrids were generated under a moderately metastatic TN BCa cell line SUM159 as the common nuclear background with mitochondria from benign breast epithelium (A1N4) and moderately metastatic (SUM159) TN BCa cells. In vitro and in vivo studies suggested that even under the common moderately cancerous nuclear background, mitochondria from benign cells inhibit and metastatic cell induce cancer properties of a moderately aggressive TN BCa cell. Gene expression studies identified c-Src onco-pathway as one of the major cancer pathways altered according to the mitochondria status of the cybrids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE72319 | GEO | 2016/04/14

SECONDARY ACCESSION(S): PRJNA293743

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profiling of transmitochondrial cybrids (triple negative breast cancer cells in SUM159 background)

Project description:We used a transmitochondrial cybrid (cybrids)-based discovery approach to identify mitochondria-regulated cancer pathways in TN BCa. Cybrids were generated under a moderately metastatic TN BCa cell line SUM159 as the common nuclear background with mitochondria from benign breast epithelium (A1N4) and moderately metastatic (SUM159) TN BCa cells. In vitro and in vivo studies suggested that even under the common moderately cancerous nuclear background, mitochondria from benign cells inhibit and metastatic cell induce cancer properties of a moderately aggressive TN BCa cell. Gene expression studies identified c-Src onco-pathway as one of the major cancer pathways altered according to the mitochondria status of the cybrids. SUM159 Ï0 cells were used as nuclear donor and A1N4 and SUM 159 cells were used as mitochondrial donor cells. The two groups were profiled for gene expression using microarrays. two group comparison (159/SUM159 vs A1/SUM159)

2016-04-14 | E-GEOD-72319 | biostudies-arrayexpress

RNA Sequencing of transmitochondrial cybrids

Project description:RNA Sequencing of transmitochondrial cybrids revealed a downregulation of oxidative phosphorylation and nicotinamide metabolism in cybrids with patient mitochondria

2022-10-19 | GSE215859 | GEO

Transcriptome profiling of cybrids cells with and without m.14487T>C mutation

Project description:Transcriptome profiling is a sensitive strategy to uncover the change of mitochondrial to nucleus signaling due to the minor alternation of mitochondrial OXPHOS function. Therefore, we hypothesized that even a minor alternation of OXPHOS function can also remold transcriptome profiles in m.14487T>C mutant cybrid when compared with control cybrids. Our data suggest that a comparable mitochondrial OXPHOS function between m.14487T and m.14487C cybrids is not because of compensatory boost of mitochondrial biogenesis in m.14487C cybrids.

2020-05-19 | GSE131739 | GEO

Mitochondrial 12S hypermethylation in HeLa cells and A1555G cybrids

2012-09-18 | GSE33780 | GEO

Mitochondrial 12S hypermethylation in HeLa cells and A1555G cybrids

Project description:This dataset investigates the transcriptional effect of mitochondrial 12S rRNA hypermethylation, both by overexpressing the mitochondrial methyltransferase mtTFB1 in HeLa cells and by using A1555G cybrids, where the 12S rRNA is hypermethylated. HeLa cells overexpressing a methyltransferase-deficient mtTFB1 (mtTFB1[G65A]) and wild-type A1555A cybrids were used as controls. four samples with 12S rRNA hypermethylation (two cell lines, with two biological replicates each) versus four samples with basal 12S rRNA methylation (two cell lines, with two biological replicates each)

2012-09-18 | E-GEOD-33780 | biostudies-arrayexpress

RNA Sequencing of transmitochondrial cybrids

Project description:RNA Sequencing of transmitochondrial cybrids

| PRJNA891041 | ENA

Crosstalk from Non-Cancerous Mitochondria Can Inbibit Tumor Properties of Metastatic Cells by Suppressing Oncogenic Pathways

Project description:Microarray analysis suggested that several oncogenic pathways observed in cybrids with cancer mitochondria are inhibited in cybrids with non-cancerous mitochondria. These results suggest the critical oncogenic regulation by motochondrial-nuclear cross talk and highlights rectifying motochondiral functional properties as a promising target in cancer therapy.

2019-03-21 | GSE128610 | GEO

Hypoxia-Induced Transcriptional Differences in African and Asian Versus European Diabetic Cybrids

Project description:We then performed gene expression profiling analysis using data obtained from RNA-seq of 16 different cybrid lines at normoxia and hypoxia conditions. The goal was to compare the impact of mitochondrial haplogroup (European vs Non-European) on gene expression in the above conditions.

2022-08-22 | GSE211611 | GEO

Arabidopsis thaliana Cybrids - RNA Sequencing

Project description:Reciprocal cybrids reveal how organellar genomes affect plant phenotypes

| PRJEB35324 | ENA

Arabidopsis thaliana Cybrids - Whole Genome Sequencing

Project description:Reciprocal cybrids reveal how organellar genomes affect plant phenotypes

| PRJEB29654 | ENA