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Gene expression profiling of MDA231 cells with alterations involving beta-oxidation pathway

ABSTRACT: In order to confirm the role of fatty acid β-oxidation in Src regulation, we performed gene expression analysis in MDA231 cells from in vivo model treated with ETX or knockdown of CPT1 or CPT2 using shRNA. As expected, inhibition of β-oxidation showed a gene expression pattern that is opposite to the published Src regulated gene pattern. The known Src up-regulated genes are down-regulated and Src down-regulated genes are up-regulated in β-oxidation inhibited cells. Western Blotting further confirmed the gene expression pattern. Knockdown of CPT1 or CPT2 inhibited Src Y416 autophosphorylation as observed with ETX.

ORGANISM(S): Homo sapiens

PROVIDER: GSE72320 | GEO | 2016/04/14

SECONDARY ACCESSION(S): PRJNA293742

REPOSITORIES: GEO

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Gene expression profiling of MDA231 cells with alterations involving beta-oxidation pathway

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Project description:Gene expression profiling of MDA231 cells with alterations involving beta-oxidation pathway

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