Transcriptomics

Dataset Information

0

RNAseq analysis of the duodenum of intestine-specific adult SD mutant mice


ABSTRACT: Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/SD;Villin-Cre (SDint) mice versus control Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq. Results: 206 genes with a p-value <0.05 were significantly changed. Among these are some enteroendocrine hormones. Conclusion: The SD domain of Nkx2.2 regulates specification of some enteroendocrine cells

ORGANISM(S): Mus musculus

PROVIDER: GSE72762 | GEO | 2016/07/28

SECONDARY ACCESSION(S): PRJNA295014

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-07-28 | E-GEOD-72762 | biostudies-arrayexpress
2016-07-28 | E-GEOD-72761 | biostudies-arrayexpress
2016-07-28 | E-GEOD-72764 | biostudies-arrayexpress
2016-07-28 | GSE72764 | GEO
2016-07-28 | GSE72761 | GEO
2016-07-28 | E-GEOD-78902 | biostudies-arrayexpress
2016-07-28 | GSE78902 | GEO
2017-01-09 | GSE80444 | GEO
2016-12-20 | GSE79724 | GEO
2016-12-20 | GSE79723 | GEO