Transcriptomics

Dataset Information

0

KRas targeting by clinically significant miR-193a-3p inhibits mutant KRas lung cancer growth, circulating tumor cells and metastasis in vivo


ABSTRACT: Ras family oncogenes are mutated in approximately 30% of human cancers and cause resistance to multiple treatment modalities. While identifying methods to directly target mutant KRas have been challenging, targeting regulators of KRas may be beneficial. Using a systems approach of integrating a genome-wide miRNA screen with patient data of a phospho-proteomic signature of the KRas downstream pathway, we identified miR-193a-3p as a potent tumor suppressor capable of reversing KRas-related signaling, whereby the 3’UTR of KRas is directly targeted via two miR-193a-3p binding sites. Mechanistic studies revealed that miR-193a-3p inhibited the KRas protein signature, KRas downstream transcriptomic network, proliferation, induced a G1 arrest, and reduced colony formation in 3D cultures through direct targeting of KRas. An ex vivo lung cancer model showed that miR-193a-3p significantly reduced the viability of circulating tumor cells as well as decreased metastasis. In vivo studies revealed that miR-193a-3p significantly reduced tumor growth as well as metastasis of a KRas-mutant lung cancer xenograft model.

ORGANISM(S): Homo sapiens

PROVIDER: GSE73194 | GEO | 2016/09/18

SECONDARY ACCESSION(S): PRJNA296353

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-01-31 | GSE185062 | GEO
2021-02-24 | GSE154080 | GEO
| EGAD00001006844 | EGA
| EGAD00001006842 | EGA
2021-09-08 | PXD013987 | Pride
2022-06-15 | GSE179399 | GEO
2015-02-05 | E-GEOD-52798 | biostudies-arrayexpress
2021-02-24 | GSE161419 | GEO
2023-09-15 | GSE229559 | GEO
2020-07-29 | GSE143326 | GEO