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HTERT promotes cell adhesion and migration independent of telomerase activity

ABSTRACT: We sequenced mRNA from 6 human cell lines stably over-expressed specific gene or empty vector, and searched for differently expressed genes after gene over-expression as compared to empty vector.

ORGANISM(S): Homo sapiens

PROVIDER: GSE73277 | GEO | 2015/09/22

SECONDARY ACCESSION(S): PRJNA296510

REPOSITORIES: GEO

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hTERT promotes cell adhesion and migration independent of telomerase activity

Project description:We sequenced mRNA from 6 human cell lines stably over-expressed specific gene or empty vector, and searched for differently expressed genes after gene over-expression as compared to empty vector. mRNA levels were compared as following pairs: U2OS+hTERT vs U2OS+vector; U2OS+hTERTmut vs U2OS+vector; VA-13+hTERT vs VA-13+vector; VA-13+hTERTmut vs VA-13+vector.

2015-09-22 | E-GEOD-73277 | biostudies-arrayexpress

hTERT promotes cell adhesion and migration independent of telomerase activity.

Project description:hTERT, a catalytic component of human telomerase, is undetectable in normal somatic cells but up-regulated in cancer and stem cells where telomere length is maintained by telomerase. Accumulated evidence indicates that hTERT may have noncanonical functions beyond telomerase by regulating the expression of particular genes. However, comprehensive identification of the genes regulated by hTERT is unavailable. In this report, we expressed WT hTERT and hTERTmut which displays dysfunctional catalytic activity, in human U2OS cancer cells and VA-13 immortalized fibroblast cells, both of which lack endogenous hTERT and hTR expression. Changes in gene expression induced by hTERT and hTERT-mut expression were determined by genome-wide RNA-seq and verified by qPCR. Our results showed that hTERT affects different genes in two cell lines, implying that the regulation of gene expression by hTERT is indirect and cell type dependent. Moreover, functional analysis identifies cell adhesion-related genes that have been changed by hTERT in both cell lines. Adhesion experiments revealed that hTERT expression significantly increases cell adhesion. Monolayer wound healing and transwell assays demonstrated increased cell migration upon hTERT expression. These results provide new evidence to support a noncanonical function for hTERT in promoting tumorigenesis.

| S-EPMC4789728 | biostudies-literature

Phenotypic characterisation of LRRN4CL over expression

Project description:This study aims to use RNAseq to identify differentially expressed transcripts in human melanoma cells that over-express the cell surface protein, LRRN4CL, relative to empty-vector control cells, to provide mechanistic insight into how LRRN4CL over-expression confers enhanced pulmonary metastatic colonisation abilities.

| EGAS00001003976 | EGA

EGAS00001003976-sc-2020-07-01T08:56:44Z - samples

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Evaluation of mRNA expression changes upon TRAF4 mis-expression in HT1376 and T24 bladder cancer cell lines

Project description:TRAF4 is highly expressed in the epithelial bladder cancer cell line, HT1376 and poorly expressed in the mesenchymal cell line, T24. We evaluated the genetic changes in HT1376 upon TRAF4 knockdown using two independent shRNAs targetting TRAF4. The changes were documented with respect to the control cell line transduced with empty vector shRNA plasmid. Also, we over-expressed (myc-tagged) TRAF4 in T24 and evaluated mRNA changes with respect to cell line over-expressing empty vector with a myc-tag.

2020-11-16 | GSE161509 | GEO

Rhodobacter sphaeroides 2.4.1 pRKSorX144 versus pRK415 under singlet oxygen stress.

Project description:To learn more about the function of SorX, we constructed an over-expression strain. For this purpose, the sorX gene together with its 28-bp upstream region was cloned into the over-expression vector pRK4352 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a SorX over-expression that is driven by the constitutive 16S rRNA (RSP_4352) promoter. The over-expression strain was compared to an empty vector control (pRK415). Total RNA from the over-expression (pRKSorX144) and from the empty vector control (pRK415) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SorX-induced changes on transcriptome level.

2017-01-01 | GSE79421 | GEO

Homo sapiens

Project description:OSLN Reprogramming cells: empty vector vs. HIF2α over-expression

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Rhodobacter sphaeroides 2.4.1 pPos19 versus pBBR under singlet oxygen stress

Project description:To learn more about the function of Pos19, we constructed an over-expression strain. For this purpose, the Pos19 gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced Pos19 over-expression compared to an empty vector control. Total RNA from the over-expression (pPos19) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate Pos19-induced changes on transcriptome level.

2017-02-09 | GSE81718 | GEO

Shotgun analysis of proteins co-immunoprecipitating with OCIAD1-FLAG

Project description:Interactome of OCIAD1-FLAG was studied. TMT-based relative quantification of protein levels was performed in eluates from mitochondria of HEK293 cells that expressed OCIAD1-FLAG vs empty vector and from mitochondria of OCIAD1-KO cells that expressed OCIAD1-FLAG vs empty vector.

2024-11-08 | PXD043863 | Pride

Using small RNA-Seq to verify that 5' tRNA-fMet1 half is over-expressed in outer membrane vesicles (OMVs)

Project description:5′ tRNA-fMet1 half (5′- CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGGGCTC-3′) was cloned into the arabinose-inducible expression vector pMQ70. PA14 was transformed with the tRNA-fMet1 half expression vector or empty vector via electroporation. Characterization of the sRNA content of OMVs secreted by 5' tRNA-fMet1 half over-expression clone and empty vector control clone.

2021-09-12 | GSE183897 | GEO

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