Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide selection and testing of superior reference genes for quantitative gene expression normalization in tobacco (Nicotiana tabacum)

ABSTRACT: To diversify the pool of reliable reference genes in tobacco, the expression stability of 44,873 tobacco genes (ESTs) were measured using a custom-designed microarray in a diverse set of 21 samples, representing various tissue types and developmental stages. A total of 14 genes were selected as candidate reference genes because they appeared to be the most stable. These genes were compared with two widely used housekeeping genes (L25 and EF-1a) for further validation by qRT-PCR

ORGANISM(S): Nicotiana tabacum

PROVIDER: GSE73440 | GEO | 2015/09/26

SECONDARY ACCESSION(S): PRJNA297025

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Profile of Animal Cell Death Gene Expressing Nicotiana tabacum

Project description:Transgenic tobacco (Nicotiana tabacum) expressing Caenorhabditis elegans cell death genes, Ced4 and Ced3, show evidence suggesting such expressions protect the plants from infestation by the plant parasitic nematode Meloidogyne incognita. Although positive results have been correlated with the gene expressions (data in preparation for publication; a draft of the publication can be provided upon request), the mechanism by which the nematode protection is manifested is not clearly understood. One possibility is that the C. elegans cell death proteins produced by the transgenic plants are being ingested and incorporated into the nematode’s own cell death pathway, leading to their demise. Alternatively, it is also possible that expression of the C. elegans cell death genes promotes the endogenous resistance genes of the plant, leading to nematode resistance. We want to test the later hypothesis by conducting a reference design microarray experiment to establish the expression profile of Ced3, and Ced4 homozygous plants and Ced3xCed4 double heterozygous plants in comparison with wild-type tobacco plants. If the hypothesis is correct, we expect to detect increased expression of pathogenicity-related genes in the transgenic plants. Furthermore, characterization of the expression profiles in these transgenic plants will provide us directionality for our future research on the elucidation of this resistance mechanism. Keywords: Reference design

2007-09-01 | GSE8277 | GEO

Nicotiana tabacum

Project description:Genome-wide selection and testing of superior reference genes for quantitative gene expression normalization in tobacco (Nicotiana tabacum)

| PRJNA297025 | ENA

Nicotiana tabacum

Project description:Induction of tobacco genes by Agrobacterium oncogene 6b

| PRJNA101169 | ENA

Profile of Animal Cell Death Gene Expressing Nicotiana tabacum

2007-09-01 | E-GEOD-8277 | biostudies-arrayexpress

Nicotiana tabacum

Project description:tobacco transcriptome sequencing

| PRJNA1057854 | ENA

Nicotiana tabacum seed lipid droplet proteome

Project description:The number of known proteins associated with plant lipid droplets (LDs) is small compared to other organelles. Many questions of LD biosynthesis and degradation remain open, also due to lack of candidate LD proteins whose characterization could help to elucidate their function in those processes. We performed a proteomic screen on LDs isolated from Nicotiana tabacum pollen tubes. Proteins that were highly enriched in the LD fraction compared to the total or cytosolic fraction where verified for LD localization via transient expression in tobacco pollen tubes. We also compared the isoforms of typical LD proteins found in the pollen tubes on a qualitative level to the isoforms found in tobacco seeds.

2018-08-13 | PXD009186 | Pride

Nicotiana tabacum

Project description:Sense transgene-induced post-transcriptional gene silencing in tobacco compromises the splicing of endogenous counterpart genes

| PRJDB1809 | ENA

Transcriptome profiling of male gametophyte development in Nicotiana tabacum

Project description:Transcriptome profiling of three developmental stages of immature male gametophyte intobacco (Nicotiana tabacum) Total RNA isolated from tobacco microspores and early and late bicellular pollen was hybridised on Agilent Tobacco Gene Expression Microarray 4x44K in two biological replicates per sample

2014-10-16 | E-GEOD-62349 | biostudies-arrayexpress

Nicotiana tabacum

Project description:Transcriptome analysis of the grafting tobacco

| PRJDB11010 | ENA

nicotiana tabacum

Project description:tobacco leaf under chilling stress Transcriptome

| PRJNA429494 | ENA