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Expression data from sorted monoyctes/macrophages

ABSTRACT: Tissue injury, such as incisional wound, results in an inflammatory response as well as acute to chronic mechanical and thermal pain. It is now understood that there is a strong contribution of these immune cells to the pain phenotype. We used microarray analysis of sorted CD11b+Ly6G- myeloid cells extracted from the mouse hindpaw 24h after incisional wound to identify distinct classes of upregulated genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE73667 | GEO | 2015/11/06

SECONDARY ACCESSION(S): PRJNA297593

REPOSITORIES: GEO

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Expression data from sorted monoyctes/macrophages

Project description:Tissue injury, such as incisional wound, results in an inflammatory response as well as acute to chronic mechanical and thermal pain. It is now understood that there is a strong contribution of these immune cells to the pain phenotype. We used microarray analysis of sorted CD11b+Ly6G- myeloid cells extracted from the mouse hindpaw 24h after incisional wound to identify distinct classes of upregulated genes. CD11b+Ly6G- myeloid cells were sorted by expression of Ly6C (high, medium, and low) and used for microarray analysis. All sorted cells were collected on the same day to reduce variability.

2015-11-06 | E-GEOD-73667 | biostudies-arrayexpress

C57BL/6 substrain differences in inflammatory and neuropathic nociception and genetic mapping of a major quantitative trait locus underlying acute thermal nociception

Project description:Sensitivity to different pain modalities has a genetic basis that remains largely unknown. The use of closely related inbred mouse strains can facilitate gene mapping of nociceptive behaviors in preclinical pain models. We previously reported enhanced sensitivity to acute thermal nociception in C57BL/6J (B6J) versus C57BL/6N (B6N) substrains. Here, we expanded on pain phenotypes and observed an increase in inflammatory nociceptive behaviors induced by hindpaw formalin injections in B6J versus B6N mice (Charles River Laboratories). No strain differences were observed in mechanical or thermal hypersensitivity or in paw diameter following the Complete Freund s Adjuvant (CFA) model of inflammatory pain, indicating specificity in the inflammatory nociceptive stimulus. In the chronic nerve constriction injury (CCI), a model of neuropathic pain, no strain differences were observed in baseline mechanical threshold or in mechanical hypersensitivity up to one month post-CCI. We replicated the enhanced thermal nociception in B6J mice in the 52.5 C hot plate test relative to B6N mice from The Jackson Laboratory. Using a B6J x B6N-F2 cross (N=164), we mapped a major QTL underlying hot plate sensitivity to chromosome 7 that peaked at 26 Mb (LOD = 3.81, 8.74 Mb-36.50 Mb) that was more pronounced in males. Genes containing expression QTLs (eQTLs) associated with the peak nociceptive marker that have been implicated in pain and inflammation include Ryr1, Cyp2a5, Pou2f2, Clip3, Sirt2, Actn4, and Ltbp4 (FDR < 0.05). Future studies involving positional cloning and gene editing will determine the quantitative trait gene(s) and potential pleiotropy of this locus across other pain modalities. RNA-seq data and genotype information from striatum punches of F2 C57BL/6J (B6J) cross C57BL/NJ (B6NJ) oxycodone-treated mice. Genotypes are given relative to B6J allele, eg 0 = homozygous B6J.

2018-11-26 | GSE119719 | GEO

Translation Regulation of the Prolactin Receptor Defines Sexually Dimorphic Pain Responses to Prolactin

Project description:Transcriptional control by gonadal hormone nuclear receptors is the foundation for sex-dependent physiological processes, including pain. Our current findings point to sex-dependent translation control as a new mechanism for sexual dimorphisms in pain. We show that hindpaw or spinal administration of exogenous prolactin (PRL) induces thermal and mechanical hyperalgesia in a female-selective fashion. Unexpectedly, PCR and transcriptomic analysis of hindpaw and dorsal root ganglion (DRG) tissues reveal no differences in PRL receptor (Prlr) mRNA between sexes. Variance in overall hindpaw and DRG transcriptomes between females and males were minor. We find that Prlr mRNA and protein is mainly expressed by peptidergic nociceptors as shown by profiling using Prlr reporter mice and electrophysiology, but there are far more peptidergic neurons with functional Prlr in females than in males. In line with this, exogenous PRL increased excitability only in female neurons. Moreover, in male DRG neurons, 6-24 hr estrogen exposure establishes PRL sensitivity in Prlr mRNA+ male neurons that do not show PRL responses under other conditions. PRL-induced behavioral hypersensitivity as well as sensitivity to PRL in female DRG neurons can be ablated by treatment with cap-dependent translation inhibitor 4EGI-1. Spinal cord immunohistochemistry of Prlr reporter mice highlight that Prlr protein is only found in female DRG neurons, but Prlr mRNA localizes to central terminals of male and female sensory neurons. Based on these findings, we propose a novel mechanism for sex-dependent regulation Prlr mRNA translation that renders female DRG neurons uniquely responsive to PRL.

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Translation Regulation of the Prolactin Receptor Defines Sexually Dimorphic Pain Responses to Prolactin

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2017-08-09 | PXD005064 | Pride

RNA-sequencing on sorted tumor-associated macrophages from vehicle or JHU083 pro-drug of 6-Diazo-5-oxo-L-norleucine (glutamine antagonist) treated 4T1 (breast cancer) tumor bearing mice

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Gene expression in sorted myeloid population from mice

Project description:Cells expressing macrophage markers (Cd11b+F4/80+CD169+CX3CR1neg) were sorted from the bone marrow of C57BL/6 mice and their gene expression profile analysed using RNA sequencing. Analysis of the most highly expressed genes revealed a gene expression signature consistent with a granulocyte population (i..e high expression of Ly6g and Cxcr2), rather than macrophages (i.e. low/negligible expression of Adgre1, Cd163 and Mertk). Collectively these data demonstrate that cells which stain positive for conventional macrophage markers exhibit a gene expression signature consistent with cells of the granulocyte lineage.

2022-01-21 | GSE186361 | GEO

Ly6C monocyte subsets transcriptome analysis from wild type and hyperhomocysteinemic mice

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