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RNA-Seq of human placenta: spontaneous preterm birth in the presence of intra-amniotic infection/inflammation and controls

ABSTRACT: Using RNA-sequencing (RNA-Seq and miRNA-Seq), we analyzed paired villous trophoblast and decidual basalis transcriptomes of 15 women pregnant with singleton gestations grouped as follows: (1) spontaneous preterm birth (PTB) in the setting of amniocentesis-proven intra-amniotic infection (IAI) and histological chorioamnionits (n=5; GA median [range]: 26 [25-31] weeks); (2) spontaneous idiopathic preterm birth (iPTB, n=5, GA: 32 [30-33] weeks); and (3) term normal pregnancy, that delivered a heathy infant by cesarean section in the absence of labor (n=5; GA: 39 [38-39] weeks).

ORGANISM(S): Homo sapiens

PROVIDER: GSE73712 | GEO | 2016/06/14

SECONDARY ACCESSION(S): PRJNA297708

REPOSITORIES: GEO

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miRNA-Seq of human placenta: spontaneous preterm birth in the presence of intra-amniotic infection/inflammation and controls

2016-06-14 | GSE73713 | GEO

miRNA-Seq of human placenta: spontaneous preterm birth in the presence of intra-amniotic infection/inflammation and controls

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Project description:Preterm birth is a multifactorial condition defined as birth less than 37 weeks of gestation. This study aimed to identify early pregnancy biomarkers from maternal genome-wide and transcriptome-wide data of women experiencing two types of spontaneous PTB (sPTB): spontaneous preterm birth (SPTB) and preterm premature rupture of membranes (PPROM). Whole blood samples were obtained from women with singleton pregnancies (N=567) recruited at the Liverpool WomenÃ¢Â€Â™s Hospital at 16 and 20 weeks of gestation. Women with a previous history of sPTB pregnancies <34 weeks were categorised on their index pregnancy outcome as spontaneous preterm birth (SPTB) and preterm premature rupture of membranes (PPROM) or high-risk term control (HTERM, Ã¢Â‰Â¥37 weeks). Women with no history of SPTB/PPROM and delivered at term were LTERM (Ã¢Â‰Â¥39 weeks of gestation). DNA was genotyped on the UK Biobank AxiomÃ¢Â„Â¢ array (Applied BiosystemsÃ¢Â„Â¢, Thermo Fisher Scientific) and RNA who processed using the ClariomÃ¢Â„Â¢ D Human assay (Thermo Fisher Scientific). Genome-wide association analysis, differential expression analysis and expression quantitative trait loci (eQTL) were completed.

| EGAS00001005076 | EGA

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