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Discovery and Differential Expression of miRNAs in Fusarium Wilt Resistant and Susceptible Banana Varieties

ABSTRACT: MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that regulate targeted mRNAs by degrading or repressing translation, considered as post-transcrption regulators. So far, a large number of miRNAs have been discovered in model plants, but little information is available on miRNAs in banana. In this study, by sequencing the small RNA (sRNA) transcriptomes of Fusarium wilt resistant and susceptible banana varieties, 139 members in 38 miRNA families were discovered, and six out of eight new miRNAs were confirmed by RT-PCR. According to the analysis of sRNA transcriptome data and qRT-PCR verification, some miRNAs were differentially expressed between Fusarium wilt resistant and susceptible banana varieties. Two hundred and ninety-nine and 31 target genes were predicted based on the draft maps of banana B genome and Fusarium oxysporum (FOC1, FOC4) genomes respectively. Specifically, two important pathogenic genes in Fusarium oxysporum genomes, feruloyl esterase gene and proline iminopeptidase gene, were targeted by banana miRNAs. These novel findings may provide a new strategy for the prevention and control of Fusarium wilt in banana.

ORGANISM(S): Musa acuminata AAA Group Musa AAB Group

PROVIDER: GSE74005 | GEO | 2016/10/14

SECONDARY ACCESSION(S): PRJNA298728

REPOSITORIES: GEO

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Project description:The soil-borne fungal pathogen Fusarium oxysporum f.sp. is responsible for Fusarium wilt. cubense tropical race 4, is one of the most devastating diseases in bananas, regarded as a major yield-reducing factor in the banana industry worldwide. Understanding the molecular interactions in banana defense responses is an important tool to reveal the unexplained processes that underlie banana resistance to Fusarium oxysporum f. sp. cubense tropical race 4. The seedlings of moderately resistant variety Guijiao No. 9 and a susceptible cultivar Guijiao No. 6 were cultured in tissue culture, and the characterize protein profile expression changes responses to after inoculation the Fusarium oxysporum f. sp. of cubense tropical race 4 were detected by isobaric labeling based on MS2 quantification at the 2nd, 4th, 6th and 8th day. Interestingly, new genes in the resistance of banana to Foc37-GFP were identified, including several other serine/threonine-protein kinase, AvrRpt-cleavage domain-containing protein, peptidylprolyl isomerase and some Jacalin-type lectin domain, the resistance-related pathways “ribosome”, “microbial metabolism in diverse environments”,“carbon metabolism”,“biosynthesis of amino acids”and “biosynthesis of antibiotics” pathways were significantly enriched, the resistant banana cultivar Guijiao 9 shows formation of different constitutive cell barriers to restrict spreading of Fusarium oxysporum f. sp. cubense tropical race 4. In this study, the dynamic change root proteomic of moderately resistant cultivar Guijiao 9 and a susceptible cultivar Guijiao 6 were characterized and provided a differentially expressed proteins comparative analysis of the compatible and incompatible interaction between Fusarium oxysporum f. sp. cubense tropical race 4 and banana. These findings provide a substantial contribution to existing sequence resources for banana, and a strong basis for future proteomic research. The proteins that displayed two-fold changes in intensity are related to biochemical processes that may be differentially altered at various times after Fusarium oxysporum f. sp. cubense tropical race 4 infection. These findings will accelerate research on resistance in banana to Fusarium oxysporum f. sp. cubense tropical race 4 and contribute to a better understanding of the banana defense mechanism to plant pathogens, hopefully.

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Discovery and Differential Expression of miRNAs in Fusarium Wilt Resistant and Susceptible Banana Varieties

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