Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of a Streptococcus pyogenes mutant lacking the eukaryote-type ser/thr kinase (SP-STK)

ABSTRACT: We report the mRNA expression profile of Streptococcus pyogenes Type M1 strain M1T15448-derived mutant lacking eukaryote-type Serine threonine kinase.

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE74590 | GEO | 2016/11/02

SECONDARY ACCESSION(S): PRJNA301045

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq analysis of a Streptococcus pyogenes mutant lacking the eukaryote-type ser/thr phosphatase (SP-STP)

Project description:We report the mRNA expression profile of Streptococcus pyogenes Type M1 strain M1T15448-derived mutant lacking Serine threonine phosphatase.

2016-10-29 | GSE74541 | GEO

RNA-seq-based transcriptome analysis of a Streptococcus pyogenes mutant expressing cytoplasmically restricted enolase.

Project description:We report the mRNA expression profile of Streptococcus pyogenes Type M1 strain M1T15448-derived mutant lacking eukaryote-type Serine threonine kinase.

2016-11-02 | GSE74638 | GEO

RNA_Seq-based global transcriptome analysis of Streptococcus pyogenes M1T15448 lacking Spy_1476/SP-TyK

Project description:We report the mRNA expression profile of Streptococcus pyogenes M1T15448 strain-derived mutant lacking the spy_1476/sp-tyk gene encoding tyrosine kinase/GTP-ATP hydrolase

2019-04-13 | GSE113381 | GEO

The role of Stk in Streptococcus pyogenes

Project description:Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221

2011-08-01 | GSE28881 | GEO

RNA-seq analysis of S. pyogenes SP-PTP mutants

Project description:We report the mRNA expression profile of Streptococcus pyogeens Type M1 strain M1T15448-derived mutant lacking phospotyrosine phosphatase.

2015-06-01 | GSE58412 | GEO

The role of Stk in Streptococcus pyogenes

Project description:Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221 The RNA prepared from triplicate cultures for the wild type and the stk mutant strains was each labeled with Cy3 and hybridized to duplicate arrays. Reference RNA consisted of pooled RNA for the wild type and stk mutant, labeled with Cy5.

2011-07-31 | E-GEOD-28881 | biostudies-arrayexpress

RNA_Seq-based global transcriptome analysis of Streptococcus pyogenes M1T15448 lacking Spy_1476/SP-TyK

Project description:RNA_Seq-based global transcriptome analysis of Streptococcus pyogenes M1T15448 lacking Spy_1476/SP-TyK

| PRJNA451018 | ENA

Transcriptome analysis of the eukaryotic-type ser/thr kinase PknB in Staphylococcus aureus

2010-03-11 | GSE15346 | GEO

<i>Streptococcus suis</i> DivIVA Protein Is a Substrate of Ser/Thr Kinase STK and Involved in Cell Division Regulation.

Project description:Streptococcus suis serotype 2 is an important swine pathogen and an emerging zoonotic agent that causes severe infections. Recent studies have reported a eukaryotic-like Ser/Thr protein kinase (STK) gene and characterized its role in the growth and virulence of different S. suis 2 strains. In the present study, phosphoproteomic analysis was adopted to identify substrates of the STK protein. Seven proteins that were annotated to participate in different cell processes were identified as potential substrates, which suggests the pleiotropic effects of stk on S. suis 2 by targeting multiple pathways. Among them, a protein characterized as cell division initiation protein (DivIVA) was further investigated. In vitro analysis demonstrated that the recombinant STK protein directly phosphorylates threonine at amino acid position 199 (Thr-199) of DivIVA. This effect could be completely abolished by the T199A mutation. To determine the specific role of DivIVA in growth and division, a divIVA mutant was constructed. The ΔdivIVA strain exhibited impaired growth and division, including lower viability, enlarged cell mass, asymmetrical division caused by aberrant septum, and extremely weak pathogenicity in a mouse infection model. Collectively, our results reveal that STK regulates the cell growth and virulence of S. suis 2 by targeting substrates that are involved in different biological pathways. The inactivation of DivIVA leads to severe defects in cell division and strongly attenuates pathogenicity, thereby indicating its potential as a molecular drug target against S. suis.

| S-EPMC5869912 | biostudies-literature

Transcriptome analysis of the eukaryotic-type ser/thr kinase PknB in Staphylococcus aureus

Project description:The function of the Staphylococcus aureus eukaryotic-like serine/threonine protein kinase PknB was investigated by transcriptome analysis using DNA-microarray technology and biochemical assays. The transcriptional profile reveals a strong regulatory impact of PknB on the expression of genes encoding proteins which are involved in purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, and glutamine synthesis. To investigate the functional role of PknB in S. aureus DNA microarray experiments were performed. In this experiment, the impact of the deletion of PknB was investigated. Each experiment was performed 5 times including a dye swap resulting in five chips per competitive comparison to increase reproducibility. All hybridizations were done with equal amounts of cDNA probes.

2010-05-05 | E-GEOD-15346 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data