Other

Dataset Information

0

Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency


ABSTRACT: The current commonly used single-guide RNA (sgRNA) structure has a shortened duplex compared with the native bacterial clustered regularly interspaced short palindromic repeats RNA (crRNA)–transactivating crRNA (tracrRNA) duplex. Here we show that modifying the sgRNA structure by extending the duplex length and mutating the fourth T of the continuous sequence of Ts (which is the pause signal for RNA polymerase III [pol III]) to C or G significantly, and sometimes dramatically, improves knockout efficiency in cells. In addition, the new sgRNA structure also significantly increases the efficiency of more challenging genome-editing procedures, such as gene deletion, which is important for inducing a loss-of-function in non-coding genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE74766 | GEO | 2015/11/17

SECONDARY ACCESSION(S): PRJNA301446

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-04-23 | E-GEOD-44582 | biostudies-arrayexpress
2013-04-23 | GSE44582 | GEO
2014-06-18 | E-GEOD-58511 | biostudies-arrayexpress
2021-04-13 | GSE171935 | GEO
| PRJNA301446 | ENA
2017-12-25 | E-MTAB-6310 | biostudies-arrayexpress
2019-01-18 | GSE114947 | GEO
2017-12-15 | E-MTAB-6357 | biostudies-arrayexpress
2017-12-15 | E-MTAB-6358 | biostudies-arrayexpress
2017-12-15 | E-MTAB-6356 | biostudies-arrayexpress