Genomics

Dataset Information

0

Hierarchy within the mammary STAT5-driven Wap super-enhancer


ABSTRACT: Super-enhancers comprise of dense transcription factor platforms highly enriched for active chromatin marks. A paucity of functional data led us to investigate their role in the mammary gland, an organ characterized by exceptional gene regulatory dynamics during pregnancy. ChIP-Seq for the master regulator STAT5, the glucocorticoid receptor, H3K27ac and MED1, identified 440 mammary-specific super-enhancers, half of which were associated with genes activated during pregnancy. We interrogated the Wap super-enhancer, generating mice carrying mutations in STAT5 binding sites within its three constituent enhancers. Individually, only the most distal site displayed significant enhancer activity. However, combinatorial mutations showed that the 1,000-fold gene induction relied on all enhancers. Disabling the binding sites of STAT5, NFIB and ELF5 in the proximal enhancer incapacitated the entire super-enhancer, suggesting an enhancer hierarchy. The identification of mammary-specific super-enhancers and the mechanistic exploration of the Wap locus provide insight into the complexity of cell-specific and hormone-regulated genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE74826 | GEO | 2016/05/31

SECONDARY ACCESSION(S): PRJNA301617

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-05-31 | E-GEOD-74826 | biostudies-arrayexpress
2018-09-18 | GSE115369 | GEO
2018-09-18 | GSE115368 | GEO
2020-03-24 | GSE145193 | GEO
2019-03-01 | GSE121438 | GEO
| PRJNA301617 | ENA
2017-05-25 | GSE92931 | GEO
2017-05-25 | GSE92587 | GEO
2017-05-25 | GSE97803 | GEO
2021-02-11 | GSE161620 | GEO