Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Primary mouse myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).

ABSTRACT: The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).

ORGANISM(S): Mus musculus

PROVIDER: GSE75222 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA302822

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mouse primary myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).

Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).

2015-11-04 | GSE74628 | GEO

Mouse cortical neuron transcriptional response to wild-type West Nile virus (WNV NY99 clone 382) and mutant virus WNV E218A [microRNA]

Project description:The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse cortical neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently. Primary cortical neurons from C57Bl/6J mouse embryos (age E15) are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WCN002' and 'WCN003') and the biological_replicate characteristic field.

2015-04-04 | E-GEOD-67474 | biostudies-arrayexpress

Mouse cortical neuron transcriptional response to wild-type West Nile virus (WNV NY99 clone 382) and mutant virus WNV E218A [microRNA]

2015-04-04 | GSE67474 | GEO

Primary mouse myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).

Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A). Mouse (C57Bl/6J) primary myeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plate in 0.8 mL R10 DC media after infection at a multiplicity of infection of 500. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 1, 8, 12 and 24 h post-infection. There were two independent biological replicates of the entire procedure. This study (WDC011) is a biological replicate of another (WDC010; GSE74628).

2015-12-31 | E-GEOD-75222 | biostudies-arrayexpress

Mouse primary myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).

Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A). Mouse (C57Bl/6J) primary amyeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plate in 0.8 mL R10 DC media after infection at a multiplicity of infection of 500. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 1, 8, 12 and 24 h post-infection. There were two independent biological replicates of the entire procedure. This study (WDC010) is a biological replicate of another (WDC011; GSE75222).

2015-11-04 | E-GEOD-74628 | biostudies-arrayexpress

Mouse cortical neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A [mRNA]

2015-04-04 | E-GEOD-67473 | biostudies-arrayexpress

Mouse granule cell neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A (mRNA)

Project description:The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse granule cell neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently. Granule cell neurons from day 6 C57Bl/6J mouse pups are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WGCN002' and 'WGCN003') and the biological_replicate characteristic field.

2015-04-29 | E-GEOD-68380 | biostudies-arrayexpress

Mouse granule cell neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A (microRNA)

Project description:The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse granule cell neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently. Granule cell neurons from day 6 C57Bl/6J mouse pups are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WGCN002' and 'WGCN003') and the biological_replicate characteristic field.

2015-04-29 | E-GEOD-68381 | biostudies-arrayexpress

Mouse cortical neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A [mRNA]

2015-04-04 | GSE67473 | GEO

Mouse granule cell neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A (mRNA)

2015-04-29 | GSE68380 | GEO