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A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation [ATAC-Seq]

ABSTRACT: We report the locations of accessible/open chromatin as assayed by ATAC-Seq on two zebrafish melanoma cell lines, zcrest 1 and zcrest 2.

ORGANISM(S): Danio rerio

PROVIDER: GSE75351 | GEO | 2016/02/01

SECONDARY ACCESSION(S): PRJNA303999

REPOSITORIES: GEO

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A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation.

Project description:The "cancerized field" concept posits that cancer-prone cells in a given tissue share an oncogenic mutation, but only discreet clones within the field initiate tumors. Most benign nevi carry oncogenic BRAF(V600E) mutations but rarely become melanoma. The zebrafish crestin gene is expressed embryonically in neural crest progenitors (NCPs) and specifically reexpressed in melanoma. Live imaging of transgenic zebrafish crestin reporters shows that within a cancerized field (BRAF(V600E)-mutant; p53-deficient), a single melanocyte reactivates the NCP state, revealing a fate change at melanoma initiation in this model. NCP transcription factors, including sox10, regulate crestin expression. Forced sox10 overexpression in melanocytes accelerated melanoma formation, which is consistent with activation of NCP genes and super-enhancers leading to melanoma. Our work highlights NCP state reemergence as a key event in melanoma initiation.

| S-EPMC4868069 | biostudies-literature

A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation

Project description:This SuperSeries is composed of the SubSeries listed below.

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