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Single-cell indexed RNA-Seq of human hematopoietic stem and progenitors

ABSTRACT: To characterize early human hematopoiesis on a single-cell level we developed an approach termed index-omics, which combines flow-cytometric, single-cell transcriptomic and single-cell lineage fate data. Healthy human bone marrow was labeled with a panel of up to 11 FACS surface markers commonly used to identify human hematopoietic stem and progenitor cells (HSPCs). Lin-CD34+38+ progenitors and Lin-CD34+CD38- stem cell enriched HSPCs were individually sorted, their surface marker fluorescence intensities recorded, and subjected to single-cell RNAseq or single-cell ex vivo cultures.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75478 | GEO | 2017/01/19

SECONDARY ACCESSION(S): PRJNA304408

REPOSITORIES: GEO

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