Transcriptomics

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Sim3, a NASP (N1/N2) related protein, escorts CENP-A for deposition at fission yeast centromeres


ABSTRACT: A defining feature of centromeres is the presence of the histone H3 variant CENP-ACnp1. It is not known how CENP-ACnp1 is specifically delivered to, and assembled into centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast we identified Sim3. Sim3 is homologous to known histone binding proteins NASPHuman and N1/N2Xenopus and aligns with Hif1S.cerevisiae to define the SHNi-TPR family. Sim3 associates with CENP-ACnp1 but is distributed throughout the nucleoplasm rather than being concentrated at centromeres. Cells defective in Sim3 function have reduced levels of CENP-ACnp1 at centromeres and display chromosome segregation defects. Newly synthesized CENPACnp1 can be deposited at centromeres by a replication-independent mechanism during G2. Sim3 is required to allow this new CENP-ACnp1 to accumulate at centromeres in S and G2-phase arrested cells. We propose that Sim3 acts as an escort which hands off CENP-ACnp1 to chromatin assembly factors, allowing its incorporation into centromeric chromatin. Keywords: Expression of sim3-143 versus wt and sim3-205 versus wt

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE7560 | GEO | 2007/11/10

SECONDARY ACCESSION(S): PRJNA100363

REPOSITORIES: GEO

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