Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effects of UV-miRNAs on melanocytes in situ

ABSTRACT: The response of microRNAs (miRNAs) to solar ultraviolet radiation (UVR) in human melanocytes while in their natural environment, and in particular, melanocytes from persons with a history of melanoma versus those from healthy persons, have yet to be described. Here, we used laser capture microdissection (LCM) to isolate and subsequently profile the expression of miRNAs in a pure population of melanocytes from human volunteers exposed to physiological doses of simulated sunlight (ssUVR). The majority of the UV-responsive miRNAs in the melanocytes of melanoma patients were down-regulated in expression when compared to those in the melanocytes of healthy individuals. De-repressed genes associated with these down-regulated UV-miRNAs belonged to regulatory modules involved in controlling the epithelial-to-mesenchymal transition (EMT), apoptosis, and immune-evasion, processes reputedly linked to melanoma.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75621 | GEO | 2016/05/24

SECONDARY ACCESSION(S): PRJNA304749

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [microarray]

2020-06-01 | GSE141618 | GEO

Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [RT-PCR]

Project description:The selection of specific miRNAs by exosomes and their release from cultured melanocytes after exposure to solar UVR (UVA+UVB) have activities in inducing these cells into a premature senescence. In this analysis, human TaqMan microRNA array cards A+B were used to measure the relative expression of miRNAs in exosomes isolated from the irradiated and un-irradiated melanocytes.

2020-06-01 | GSE141619 | GEO

RNA-seq of UV irradiated human epidermal melanocytes from neonatal foreskin, HEMn-DP2.

Project description:UVR is the principal risk factor for melanoma development. While the role of UVR in DNA mutagenesis is incontrovertible, it remains controversial what role UVR-induced mutations play in melanoma genesis. To better understand how UVR is contributing to melanoma development, we investigated the non-mutational effect of UVR on the epigenome, specifically DNA methylation. Aberrant DNA methylation changes are a hallmark in melanoma; however, there are few reports on the effects of UVR on DNA methylation. We exposed melanocytes to UVR and cultured them for one-month to detect hertible and stable changes in DNA methylation. We found both hyper and hypo methylated sites after UVR exposure. While many of these changes occured outside of promoters and areas of active gene expression, there were changes in promoter DNA methylation changes that correlated with changes in gene expression. These changes also correlated with those found in melanoma and UVR sensitive sites were prognostic of patient overall survival. Our work shows for the first time UVR induced DNA methylation changes in melanocytes and may be another way in which UVR contributes to melanoma development.

2021-03-23 | GSE169382 | GEO

DNA methylation analysis by DREAM of UV irradiated melanocytes

Project description:Ultraviolet radiation (UVR) is the greatest risk factor for melanoma development. While the role of UVR in DNA mutagenesis is generally accepted, the role of UVR-induced mutations in melanomagenesis remains controversial. To understand better how UVR is contributing to melanoma development, we investigated the non-mutational effect of UVR on the epigenome, specifically DNA methylation. Aberrant DNA methylation changes are a hallmark in melanoma and there are few reports on the effects of UVR on DNA methylation. We exposed melanocytes to UVR and cultured them for one-month to detect heritable and stable changes in DNA methylation. We found both hyper and hypo methylated sites after UVR exposure. While many of these changes occurred outside of promoters and areas of active gene expression, there were changes in promoter DNA methylation changes that correlated with changes in gene expression. These changes also correlated with those found in melanoma and UVR sensitive sites were prognostic of patient overall survival. Our work shows UVR-induced DNA methylation changes in melanocytes and may be a novel non-mutational mechanism in which UVR contributes to melanoma development.

2024-03-25 | GSE169695 | GEO

DNA methylation analysis by RRBS of UV irradiated human epidermal melanocytes from neonatal foreskin, HEMn-DP2.

Project description:UVR is the greastest risk factor for melanoma develoment. While the role of UVR in DNA mutagenesis is incontrovertable, is is unclear how UVR induced mutations contribute to melanoma. For example, many of the driver mutations ( BRAF V600E or NRAS Q61R) do not bear the UVR signature C>T mutation. To better understand how UVR is contributing to melanoma development, we investigated the non-mutational effect of UVR on the epigenome, specifically DNA methylation. Aberrant DNA methylation changes are a hallmark in melanoma and there are few reports on the effects of UVR ion DNA methylation. We exposed melanocytes to UVR and cultured them for one-month to detect hertible and stable changes in DNA methylation. We found both hyper and hypo methylated sites after UVR exposure. While many of these changes occured outside of promoters and areas of active gene expression, there were changes in promoter DNA methylation changes that correlated with changes in gene expression. These changes also correlated with those found in melanoma and UVR sensitive sites were prognostic of patient overall survival. Our work shows for the first time UVR induced DNA methylation changes in melanocytes and may be another way in which UVR contributes to melanoma development.

2021-04-05 | GSE169479 | GEO

Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes

Project description:Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes

| PRJNA594056 | ENA

Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [microarray]

Project description:Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [microarray]

| PRJNA594060 | ENA

Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts [I]

Project description:Skin damage from solar ultraviolet radiation (UVR) accumulates in the dermal extracellular matrix (ECM) and contributes to photoaging. Following UVR exposure, matrix metalloproteinases (MMPs) are secreted by dermal fibroblasts to repair and remodel the ECM. Molecular signaling pathways delineating the induction of MMPs are currently well-defined; however, the effects of UV exposure on epigenetic mechanisms of MMP induction are not as well understood. An epigenetic mechanism would further describe how MMP genes are regulated in response to UV. In this study, we examined solar simulated UVR (ssUVR)-induced gene expression changes and alterations to histone methylation in the promoters of MMP1 and MMP3 in primary human dermal fibroblasts (HDF). This set of gene expression data was generated to identify photoaging related genes (including MMP) that were impacted by ssUVR exposure in our system.

2014-08-27 | GSE60794 | GEO

Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts [II]

2014-08-27 | GSE60795 | GEO

Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [RT-PCR]

Project description:Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [RT-PCR]

| PRJNA594058 | ENA