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Transcriptomic profiling of human placental trophoblasts in response to Enterococcus faecalis invasion

ABSTRACT: Analysis of the molecular mechanisms of the interaction between E. faecalis and host placental barrier at gene expression level. The hypothesis tested in the present study was that E. faecalis influence the ranscriptomic profiling of human placental trophoblasts. Results provide important information of the response of human placental trophoblasts to E. faecalis, such as Several representative terms of the differentially expressed genes are involved in apoptosis, stress and stimulus response, placenta and embryonic development, immune response, and cell adhesion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75626 | GEO | 2015/12/03

SECONDARY ACCESSION(S): PRJNA304764

REPOSITORIES: GEO

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Project description:The bacterial pathogen Listeria monocytogenes breaches the placental barrier and infects the placental/fetal unit resulting in poor pregnancy outcomes. L. monocytogenes is thought to enter the placenta by infection of trophoblasts at the maternal/fetal interface. Trophoblasts are fetal epithelial cells that delineate the maternal/fetal interface and ensure critical functions by promoting all nutritional and waste exchanges between maternal and fetal bloods and by forming a protective immune barrier. In this work, we report the first transcriptomic analysis of L. monocytogenes-infected trophoblasts by RNA sequencing. Primary human trophoblasts (PHT) and BeWo (fused and non-fused) were infected for 5 h with WT L. monocytogenes and highly purified RNA was extracted from infected and control cells. A 200 ng total RNA sample was used for sequencing on Illumina NovaSeq SP flowcell in paired-end 150 bp format to a read yield between 70-80 million reads. Pathway analysis showed that infection upregulated TLR2-, NOD-like, and cytosolic DNA sensing pathways, as well as downstream pro-inflammatory circuitry (NF-KB, AP-1, IRF4, IRF7) leading to the production of mediators known to elicit the recruitment and activation of maternal leukocytes (IL8, IL6, TNFA, MIP-1). Signature genes associated with poor pregnancy outcomes were also upregulated upon infection. Measuring the release of 54 inflammatory mediators confirmed the transcriptomic data and revealed sustained production of tolerogenic factors (IL-27, IL-10, IL-1RA, TSLP) despite infection. Both the SYN and mononuclear trophoblasts produced cytokines, but surprisingly, some cytokines were predominantly produced by the SYN (IL-8, IL-6) or by non-fused trophoblasts (TNFA). Collectively, our data support that trophoblasts act as placental gatekeepers that limit and detect L. monocytogenes infection resulting in a pro-inflammatory response, which may contribute to the poor pregnancy outcomes if the pathogen persists.

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Transcriptomic profiling of human placental trophoblasts in response to Enterococcus faecalis invasion

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