Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression

ABSTRACT: Sexual differentiation of malaria parasites into gametocytes in the vertebrate host and subsequent gamete fertilisation in mosquitoes is essential for the spreading of the disease. The molecular processes orchestrating these transitions are far from fully understood. Here we report the first transcriptome analysis of male and female Plasmodium falciparum gametocytes coupled with a comprehensive proteome analysis. In male gametocytes there is an enrichment of proteins involved in the formation of flagellated gametes; proteins involved in DNA replication, chromatin organisation and axoneme formation. On the other hand, female gametocytes are enriched in proteins required for zygote formation and functions after fertilisation; protein-, lipid- and energy-metabolism. Integration of transcriptome and proteome data revealed 512 highly expressed maternal transcripts without corresponding protein expression indicating large scale translational repression in P. falciparum female gametocytes for the first time. Despite a high degree of conservation between Plasmodium species, 260 of these ‘repressed transcripts’ have not been previously described. Moreover, for some of these genes, protein expression is only reported in oocysts and sporozoites indicating that repressed transcripts can be partitioned into short- and long-term storage. Finally, these data sets provide an essential resource for identification of vaccine/drug targets and for further mechanistic studies.

ORGANISM(S): Plasmodium falciparum

PROVIDER: GSE75795 | GEO | 2016/06/28

SECONDARY ACCESSION(S): PRJNA305391

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression

Project description:Sexual differentiation of malaria parasites into gametocytes in the vertebrate host and subsequent gamete fertilisation in mosquitoes is essential for the spreading of the disease. The molecular processes orchestrating these transitions are far from fully understood. Here we report the first transcriptome analysis of male and female Plasmodium falciparum gametocytes coupled with a comprehensive proteome analysis. In male gametocytes there is an enrichment of proteins involved in the formation of flagellated gametes; proteins involved in DNA replication, chromatin organisation and axoneme formation. On the other hand, female gametocytes are enriched in proteins required for zygote formation and functions after fertilisation; protein-, lipid- and energy-metabolism. Integration of transcriptome and proteome data revealed 512 highly expressed maternal transcripts without corresponding protein expression indicating large scale translational repression in P. falciparum female gametocytes for the first time. Despite a high degree of conservation between Plasmodium species, 260 of these ârepressed transcriptsâ have not been previously described. Moreover, for some of these genes, protein expression is only reported in oocysts and sporozoites indicating that repressed transcripts can be partitioned into short- and long-term storage. Finally, these data sets provide an essential resource for identification of vaccine/drug targets and for further mechanistic studies. Examining the transcriptome of p47-mCherry positive female and pDynGFP positive (or double positive) male gametocytes by RNA-seq.

2016-06-28 | E-GEOD-75795 | biostudies-arrayexpress

Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes

Project description:Investigation of overall expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-30 | GSE77398 | GEO

Expression analysis of Plasmodium falciparum male and female gametocytes

Project description:Investigation of whole genome gene expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-28 | GSE77316 | GEO

The P. falciparum orthologue of Male Development Protein 3 is a Male-associated regulator of translation initiation or enhancement [RNA-Seq]

Project description:Sex determination and development of Plasmodium gametocytes is critical to the transmission of malaria parasites and involves a complex interplay of multiple molecular factors. Here, we characterized the P. falciparum gene syntenic to P. berghei MD3- pf3d7_0315600. This protein contains a non-classical CCCH zinc-finger domain and Lysine-Proline rich domain associated with ribosome receptors, suggesting a role in mRNA regulation. Disruption of PfMD3 causes a pronounced defect in male gametogenesis which severely decreases parasite transmission to mosquitoes but does not affect female gamete production. We show that abrogation of PfMD3 significantly decreases the expression of male gamete markers on the transcriptome and proteome level but also affects regulators of translation. We further investigated the function of PfMD3-3xHA using in vivo using crosslinked RNA-Protein interaction capture followed by RNA-sequencing (CLIP-seq) to determine its RNA targets and IP-MS to determine protein interactors. We found 143 bound mRNA transcripts in early-stage gametocytes and 86 transcripts in late-stage gametocytes including arid/md4 andp230, not1-g, spm3 and morc mRNA bound at both stages. Interestingly, these CLIP targets decrease significantly in the ΔPfMD3 proteome, suggesting a role for PfMD3 in translational processing. IP-MS using a PfMD3-3xHA-tagged line further confirmed this role, showing an enrichment of proteins involved in mRNA metabolism. We then propose that PfMD3 functions as a Male Associated Regulator of Translation Initiation or Enhancement (MARTIE) in the human malaria parasite P. falciparum.

2024-09-01 | GSE267361 | GEO

The proteomes of plasmodium falciparum mature male and female gametocytes

Project description:Gametocytes are obligate for the transmission of malaria parasite to mosquito. This study reports the first comprehensive proteomes of accurately separated mature male and female Plasmodium falciparum gametocytes using a transgenic line. the proteomes revealed significant differences that are consistent with the divergent functions of the two sexes. While the male proteome is enriched in proteins associated with flagella and genome replication, the female proteome is more abundant in proteins involved in metabolism, translation and organellar functions.

2017-01-30 | PXD003556 | Pride

Transcription profiling of sexually proficient and deficient clones of Plasmodium falciparum during induction of gametocytogenesis

Project description:A genome-wide expression analysis was undertaken to identify novel genes specifically activated from early stages of gametocytogenesis in Plasmodium falciparum. A comparative analysis was conducted on sexually induced cultures of reference parasite clone 3D7 and its gametocyteless derivative clone F12. Competitive hybridisations on long-oligomer microarrays representing 4488 P. falciparum genes identified a remarkably small number of transcripts differentially produced in the two clones. Upregulation of the mRNAs for the early gametocyte markers Pfs16 and Pfg27 was however readily detected in 3D7, and such genes were used as reference transcripts in a comparative time course analysis of 3D7 and F12 parasites between 30 and 40 h post-invasion in cultures induced to enter gametocytogenesis. One hundred and seventeen genes had expression profiles which correlated to those of pfs16 and pfg27, and Northern blot analysis and published proteomic data identified those whose expression was gametocyte-specific. Immunofluorescence analysis with antibodies against two of these gene products identified two novel parasite membrane associated, sexual stage-specific proteins. One was produced from stage I gametocytes and the second showed peak production in stage II gametocytes. The two proteins were named Pfpeg-3 and Pfpeg-4, for P. falciparum proteins of early gametocytes.

2007-09-26 | E-MEXP-1250 | biostudies-arrayexpress

Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte

Project description:Following fertilisation, translation of stored, but previously translationally repressed transcripts, is instrumental in shaping the proteome of the early embryo of multicellular eukaryotes; further development relies on de novo transcription of the zygotic genome. A related process was evidenced in the unicellular protozoa Plasmodium berghei during transmission to the mosquito host where fertilisation and zygote development result in the formation of an entirely new cell type, the ookinete. DOZI and CITH are mRNP components that maintain mRNAs silent in the female precursor cell. In the absence of DOZI or CITH, hundreds of transcripts are destabilised, which does not block fertilization upon transmission to the mosquito host, but impairs further development of the malaria parasite. However, how many transcripts are bound by DOZI/CITH in these mRNPs and what are they remain unknown. Here, we investigated the extent of maternal contribution during post-fertilization development of P. berghei by RNA immunoprecipitation (RIP)-Chip data mining and provided further functional significance using gene tagging and gene knockout approaches. We provide conclusive evidence that in the rodent malaria parasite Plasmodium berghei 50% of the transcriptome of the female sexual precursor cell is stably associated with the translational repressors DOZI and CITH allowing morphogenesis of the zygote into the ookinete to proceed in the absence of RNA polymerase II transcription. Using in situ tagging with GFP we find for the first time novel genes that rely on translational repression on the 5' UTR. This maternal repressome provides the developing ookinete with coding potential for key molecules required during developmental progression: a near complete set of factors required for the gliding motility apparatus, but also protein trafficking, adhesins, proteases and other factors.

2014-10-23 | E-MTAB-2900 | biostudies-arrayexpress

PFNF54-Pfs16-GFP-LUC gametocyte time course from commitment to maturity

Project description:Plasmodium falciparum gametocytes undergo 14 days of gametocytogenesis to generate mature, transmissible gametocytes. This timecourse delineates the transcriptional profiles of gametocytes from commitment to maturity.

2018-04-30 | GSE104889 | GEO

A seven-helix protein acts as a key regulator of translation during human-to-mosquito transmission of Plasmodium falciparum

Project description:Plasmodium falciparum is a unicellular parasite responsible for the majority of 440,000 death due to malaria every year. Due to their essential role for malaria transmission, gametocytes represent prime targets for transmission-blocking strategies intended to prevent spread of the deadly disease. In this study, we explored the signaling pathways leading to gametogenesis and identified a hitherto unknown protein, which structurally belongs to the class of seven-helix proteins and which thus was termed 7-helix-1. The protein is specifically expressed in female gametocytes and gene disruption leads to impaired gamete formation and thus reduced transmission of malaria parasites to mosquitoes. The loss of 7-helix-1 caused significant changes in the expression of components of the molecular machinery needed by eukaryotic cells to synthesize proteins. We thus propose that 7-helix-1 is a key regulator needed to coordinate the increased need of proteins at the onset of gametogenesis.

2018-02-23 | GSE103012 | GEO

Single-cell transcriptomics reveals transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis

Project description:The Apicomplexa constitute a large phylum of parasitic protozoans with complex life cycles that typically include meiotic sex. The life cycle of the malaria parasite, Plasmodium falciparum, includes obligate transition and stage development between a human and mosquito host. Asexual parasite replication in the human erythrocytes is followed by differentiation which leads to the formation of a precursor gamete stage, referred to as gametocytes. The gametocyte stage is solely responsible for malaria transmission into the mosquito vector where gamete fusion followed by meiosis occurs. How the parasite differentiates into male and female gametocytes in the absence of sex chromosomes largely remains an open question. Here, we combine FACS-based cell enrichment of a gametocyte reporter line followed by single-cell RNA-seq, to enable targeted characterization of the entire gametocyte developmental stage. Our data defines differential transcription programs during male and female gametocyte development and highlights a bifurcation point for sexual cell fate. We perform prediction analyses of novel candidate driver genes underlying P. falciparum sexual cell fate. Additionally, we delineate the timing of expression of members of the ApiAP2 family of transcription factors and predict their specificity in male or female P. falciparum gametocyte development.

2024-05-31 | GSE226145 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data