Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Influence of RNA modification on microRNAs

ABSTRACT: Gene expression profiles of methylated or nonmethylated miRNA oligonucleotides transfected DICER exon 5-disrupted RKO cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75927 | GEO | 2017/07/01

SECONDARY ACCESSION(S): PRJNA305727

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Influence of RNA modification on microRNAs (ver 2)

Project description:Gene expression profiles of methylated or nonmethylated miRNA oligonucleotides transfected DICER exon 5-disrupted RKO cells.

2017-07-01 | GSE83740 | GEO

Effect for target mRNA degradation by methylated microRNAs

Project description:To confirme the function of methylated micro RNAs (miR-17 and let-7a), we transfected methylated or non-methylated microRNAs to DICER KO HCT116 cell line. mRNA were isolated at 48 houes after transfection. This data show that the function were different between methylated or non-methylated microRNAs.

2018-08-25 | GSE119013 | GEO

Target suppression effect by methylated miRNA

Project description:To confirme the difference of target supression effect, we transfected non-methylated micro RNA or methylated microRNA in DICER knockout HCT116 cell.

2019-07-25 | GSE134794 | GEO

Rnase1 responsive miRNA

Project description:To determine the importance of RNase1 on cellular miRNA function, we used a lentivirus system to stably express RNase1 in dicer wt and dicer ex5 RKO cells in serum free culture. RNase1 was found to govern miRNA that reside outside the pureview of Dicer. Assignment of RNase1 as miRNA-generating enzyme suggests that, like Dicer, RNaseA enzymes mediate essential physiological functions through regulatory RNA. Two-condition experiment, wild-type RNase1 vs mutant (6x) RNase1,in two cell lines RKO dicer wild-type and RKO dicer mutant (ex5). Biological replicates: 2 for each condition and each cell line . One replicate per array.

2015-09-15 | E-GEOD-72796 | biostudies-arrayexpress

Rnase1 responsive miRNA

2015-09-15 | GSE72796 | GEO

Dicer knockout induced altered miRNA profile

Project description:We detected whole genome microarray expression profiling in three pairs of Dicer knockout coloretal cell lines and their normal controls, including DLD-1, RKO and HCT116.

2017-01-06 | GSE93177 | GEO

Comprehensive gene expression changes by the expression levels of Dicer protein

Project description:MicroRNA regulates gene expression. In this experiment, we determined the gene expression changes by the microRNA expression status using Dicer protein nulll. Wildtype, and intermediate expression. Dicer gene disrupted HCT116 colon cancer cells were transduced with tetracycline regulated Dicer protein expression. To determine the gene expression changes dependent on the Dicer protein levels, we used Dicer disrupted cells, Dicer wild-type cells, and cells with intermediate expression level of Dicer protein for the cDNA microarrya analyses.

2016-08-23 | E-GEOD-85909 | biostudies-arrayexpress

Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

2011-01-10 | GSE25310 | GEO

Methylation analysis of normal lymphocytes, HCT116, RKO and SW480

Project description:Methylation analysis of normal lymphocytes, HCT116, RKO and SW480 In vitro methylated DNA (IVD) generated from normal lymphocytes, HCT116, RKO and SW480 genomic DNA was subjected to sodium bisulfite treatment and the DNA was analyzed for CpG methylation using the Infinium Methylation Array.

2013-08-30 | E-GEOD-50511 | biostudies-arrayexpress

Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

Project description:MicroRNAs (miRNAs) are a large family of 19-22nt non-coding RNAs that post-transcriptionally regulate their mRNA targets. Computational algorithms predict that over half of all genes are regulated by miRNAs, yet approaches for experimental identification of miRNA binding sites are now emerging. To directly identify endogenous miRNA binding sites, we performed photo-crosslinking immunoprecipitation using antibodies against Ago2, followed by deep-sequencing of RNA tags (CLIP-seq) in mouse embryonic stem cells (mESCs). We also performed parallel CLIP-seq in Dicer null mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was mediated by miRNAs. We include the exon-array expression data obtained from three sets of Dicer WT and Dicer Null mESCs.These data are used to determine genes that are differentially expressed between Dicer WT and Dicer Null conditions. Six samples (3 Dicer wild-type CLIP RNA libraries representing two biological replicates, 2 Dicer null CLIP RNA libraries, 1 short-RNA library from Dicer wild-type mESCs) were analyzed. Six total mESC samples were analyzed (3 Dicer WT, 3 Dicer Null). Expression values for probesets were summarized into a single per-gene value. The log fold change for Dicer_WT/Dicer_Null was defined as the difference between the mean expression in Dicer WT mESCs and the mean expression in the Dicer Null mESCs.

2011-01-10 | E-GEOD-25310 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data