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Small RNA transcriptome in distinct cell types of the jejunal intestinal epithelium of germfree and conventionalized mice

ABSTRACT: We evaluated miRNA expression profiles by next-generation high-throughput small RNA-sequencing in distinct IEC subtypes of germ-free (GF) and conventionalized (CV) mice. We used the highly characterized Sox9-EGFP transgenic mouse model, which permits the isolation and analysis of four distinct IEC populations using fluorescence-activated cell sorting (FACS) based on differing levels of cellular EGFP intensity. These are Sox9-EGFP Low (actively cycling IESCs), Sox9-EGFP Sublow (progenitor cells), Sox9-EGFP Neg (mostly differentiated enterocytes as well as goblet cells and Paneth cells), and Sox9-EGFP High (primarily EECs).

ORGANISM(S): Mus musculus

PROVIDER: GSE76179 | GEO | 2017/01/04

SECONDARY ACCESSION(S): PRJNA306526

REPOSITORIES: GEO

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