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Gene expression in Aspergillus nidulans at different developmental stages

ABSTRACT: Microarray analysis was used to compare different developmental stages of the filamentous fungus Aspergillus nidulans. Cells were grown for various times and comparisons made between: 1. Isolated conidia (0 hours) and isotropically expanding cells (3 hours). 2. Isotropically expanding cells (3 hours) and polarly extending cells (5 hours). 3. Undifferentiated vegetative hyphae (18 hours) and synchronously conidiating cultures (42 hours). Keywords: Developmental stage comparison

ORGANISM(S): Aspergillus nidulans

PROVIDER: GSE7698 | GEO | 2008/05/01

SECONDARY ACCESSION(S): PRJNA99463

REPOSITORIES: GEO

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Gene expression in Aspergillus nidulans at different developmental stages

Project description:Microarray analysis was used to compare different developmental stages of the filamentous fungus Aspergillus nidulans. Cells were grown for various times and comparisons made between: 1. Isolated conidia (0 hours) and isotropically expanding cells (3 hours). 2. Isotropically expanding cells (3 hours) and polarly extending cells (5 hours). 3. Undifferentiated vegetative hyphae (18 hours) and synchronously conidiating cultures (42 hours). A total of 4 hybridizations were performed for each microarray experiment described in the summary. The following replicates were carried out: 1. Two biological replicates were performed using cultures grown in parallel. 2. Two Technical ‘dye swap’ replicates were carried out for each biological replicate.

2008-05-01 | E-GEOD-7698 | biostudies-arrayexpress

Gene expression in Aspergillus fumigatus at different developmental stages

Project description:Microarray analysis was used to compare different divelopmental stages of the filamentous fungi Aspergillus fumigatus af293. Cells were grone for various times and comparisons made between: 1. Dormant conidia (0 hours) and isotropically expanding cells (1hr). 2. Dormant conidia (0 hours) and isotropically expanding cells (2hr) . 3. Dormant conidia (0 hours) and isotropically expanding cells (4hr). 4. Isotropically expanding cells (4hr) and polarity extending cells (6hr). 4. Isotropically expanding cells (4hr) and germ tube (8hr).

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RNA-seq of limmmunocompetent mice in response to Aspergillus fumigatus conidia and hyphae

Project description:We bulit a pulmonary aspergillosis model in immunocompetent mice. Mice were inoculated intratracheally with 50 μL suspension of conidia , hyphal fragments or normal saline, respectively, after anesthesia. Lungs from were harvested and ground for RNA extraction 24 hours after fungal inoculation. The result of RNA-seq indicated both conidia and hyphae can activate a series of immunologically relevant pathways and these pathways were much more strongly activated by hyphae. Hyphae could promote Th2 cell differentiation and suppress Th1 cell differentiation. Both hyphae and conidia could activate Th17 cell differentiation.

2021-09-15 | GSE183979 | GEO

Crosstalk between Three Fungus-specific Transcription Factors, WetA, VosA, and VelB, in Asexual Spores of Aspergillus nidulans [RNA-seq]

Project description:In filamentous fungi, asexual sporulation involves morphological differentiation and metabolic changes. The process of asexual spore formation is tightly regulated by a variety of transcription factors including VosA, VelB, and WetA. A number of studies have demonstrated that these three transcription factors are key regulators of asexual spore formation and maturation in the model filamentous fungus Aspergillus nidulans. To gain a more mechanistic view of the roles these transcription factors play in asexual spores, genome-wide and metabolomic analyses were conducted in A. nidulans conidia. RNA sequencing and chromatin immunoprecipitation-based sequencing data suggested that the three transcription factors directly or indirectly regulate the expression of genes associated with spore-wall integrity, asexual development, and secondary metabolism. In addition, metabolomics analysis of conidia extracts showed strikingly different primary and secondary metabolite profiles for wild-type and mutant conidia. These results suggest that WetA, VosA, and VelB play key roles in the morphological development of and metabolic changes in conidia. This entry is for the RNA-seq data.

2021-01-11 | GSE154639 | GEO

Crosstalk between Three Fungus-specific Transcription Factors, WetA, VosA, and VelB, in Asexual Spores of Aspergillus nidulans [ChIP-seq]

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Drosophila melanogaster selection lines confronted with Aspergillus nidulans

Project description:Experimental evolution was conducted using Drosophila melanogaster populations that developed as larvae on breeding substrate that was infested with Aspergillus nidulans wild type, A. nidulans toxin-impaired mutant strain delta-laeA, the mycotoxin sterigmatocystin, or on fungi and toxin free substrate. Overall population were reared under these conditions for 11 generations, where after each confrontation generation one relaxation generation (fungi and toxin free breeding substrate) was conducted. Nine generations after the last selection treatment, first instar larvae were confronted with 3 days old A. nidulans wild type colonies or control conditions. 24 hours after confrontation start larvae were collected. For each biological replicate 52 larvae were collected from 4 independent confrontation units, balanced design. Three populations per selection regime were conducted, resulting in: 2 conditions x 4 selection regimes x 3 biological replicates (equal to fly population) = 24 samples. Selection regimes: sCO= control; sWT= A. nidulans wild type; sLA= A. nidulans mutant strain; sST= Sterigmatocystin. confrontation condition: cCO= control; cWT= A. nidulans wild type.

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Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours

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Project description:Although tyrosol is a quorum-sensing molecule of Candida species, it has antifungal activity at supraphysiological concentrations. Here, we studied the effect of tyrosol on the physiology and genome-wide transcription of Aspergillus nidulans to gain insight into the background of the antifungal activity of this compound. Tyrosol efficiently reduced germination of conidia and the growth on various carbon sources at a concentration of 35 mM. The growth inhibition was fungistatic rather than fungicide on glucose and was accompanied with downregulation of 2199 genes related to e.g. mitotic cell cycle, glycolysis, nitrate and sulphate assimilation, chitin biosynthesis, and upregulation of 2250 genes involved in e.g. lipid catabolism, amino acid degradation and lactose utilization. Tyrosol treatment also upregulated genes encoding glutathione-S-transferases (GSTs), increased specific GST activities and the glutathione (GSH) content of the cells, suggesting that A. nidulans can detoxify tyrosol in a GSH-dependent manner even though this process was weak. Tyrosol did not induce oxidative stress in this species, but upregulated “response to nutrient levels”, “regulation of nitrogen utilization”, “carbon catabolite activation of transcription” and “autophagy” genes. Tyrosol may have disturbed the regulation and orchestration of cellular metabolism, leading to impaired use of nutrients, which resulted in growth reduction.

2023-08-22 | GSE223010 | GEO

Degradation of intracellular glutathione in Aspergillus nidulans

Project description:Glutathione (GSH) is an abundant and widely distributed antioxidant in fungi. Hence, understanding cellular GSH metabolism is of vital importance to deciphering redox regulation in these microorganisms. In this study, we generated dugB (AN1879), dugC (AN1092), and dugB dugC double deletion mutants which display disruption of the GSH degradation pathway in Aspergillus nidulans. Deletion of dugB, dugC or both resulted in a moderate increase in GSH content under growing conditions and substantially slowed down the depletion of GSH pools under carbon starvation. Inactivation of dug genes caused reduced accumulation of reactive oxygen species, decreased autolytic cell wall degradation and extracellular enzyme production, increased sterigmatocystin formation but decreased viability in starving cultures. Changes in the transcriptomes suggested that enzyme secretions were controlled at post transcriptional level. In contrast, secondary metabolite production was also regulated at the level of mRNA abundance. Based on these findings, we suggest that GSH connects starvation and redox regulation to each other: A. nidulans cells utilize GSH as stored carbon source during starvation. The reduction of GSH contents of cells alters the redox state activating regulatory pathways responsible for carbon starvation stress responses. Under glucose rich conditions, inactivation of dug genes reduced conidia production of surface cultures, disturbed sexual development and down-regulated the transcription of genes encoding MAP kinase pathway proteins (e.g. steC, sskB, pbsA, hogA, mkkA) or proteins involved in the regulation of conidiogenesis or sexual differentiation (e.g. flbA,C,E, nosA, rosA, nsdC,D). These finding indicates that the authority of redox regulation goes far beyond the protection against redox stress; it affects development, stress responses (other than redox stress) and secondary metabolism as well.

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