Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy (RNA-seq)

ABSTRACT: We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, ago1d, mmi1d and dcr1d strains.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE77047 | GEO | 2017/02/25

SECONDARY ACCESSION(S): PRJNA309328

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy (ChIP-chip)

Project description:Occupancy profiling of lysine 9 dimethylated histone H3 in fission yeast. Occupancy profiling of Psc3, Rec8, Rad21 and Rec11 proteins in fission yeast.

2017-02-25 | GSE77015 | GEO

Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy.

Project description:Uniparental disomy (UPD), in which an individual contains a pair of homologous chromosomes originating from only one parent, is a frequent phenomenon that is linked to congenital disorders and various cancers. UPD is thought to result mostly from pre- or post-zygotic chromosome missegregation. However, the factors that drive UPD remain unknown. Here we use the fission yeast Schizosaccharomyces pombe as a model to investigate UPD, and show that defects in the RNA interference (RNAi) machinery or in the YTH domain-containing RNA elimination factor Mmi1 cause high levels of UPD in vegetative diploid cells. This phenomenon is not due to defects in heterochromatin assembly at centromeres. Notably, in cells lacking RNAi components or Mmi1, UPD is associated with the untimely expression of gametogenic genes. Deletion of the upregulated gene encoding the meiotic cohesin Rec8 or the cyclin Crs1 suppresses UPD in both RNAi and mmi1 mutants. Moreover, overexpression of Rec8 is sufficient to trigger UPD in wild-type cells. Rec8 expressed in vegetative cells localizes to chromosomal arms and to the centromere core, where it is required for localization of the cohesin subunit Psc3. The centromeric localization of Rec8 and Psc3 promotes UPD by uniquely affecting chromosome segregation, causing a reductional segregation of one homologue. Together, these findings establish the untimely vegetative expression of gametogenic genes as a causative factor of UPD, and provide a solid foundation for understanding this phenomenon, which is linked to diverse human diseases.

| S-EPMC5567995 | biostudies-literature

Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-02-25 | GSE77050 | GEO

Genome-wide map of RNA-Seq in fission yeast Schizosaccharomyces pombe

Project description:RNA-Seq from 3 different strains of fission yeast Schizosaccharomyces pombe.

2020-05-26 | GSE148191 | GEO

Genome wide map of H3K9me2 in fission yeast Schizosaccharomyces pombe.

Project description:Genome wide map of H3K9me2 in 4 different strains of fission yeast Schizosaccharomyces pombe.

2019-01-03 | GSE119604 | GEO

Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe

Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains

2013-07-20 | GSE42848 | GEO

Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis

Project description:This RNA-Seq analysis compares gene expression of fission yeast (Schizosaccharomyces pombe) strains prior to phosphate starvation (0 HR) and at various times after phosphate starvation (4, 8, 12, 24, 36, and 48 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate replete conditions (WT 0HR)

2023-01-19 | GSE213459 | GEO

Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe

Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains Examination of a single histone modification in 4 different fission yeast strains

2013-07-20 | E-GEOD-42848 | biostudies-arrayexpress