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SMARCA4 (Brg) ATPase mutations induce increased occupancy and activity of Polycomb repressive factors on chromatin

ABSTRACT: We generated a library of Brg variants with mutations in conserved regions of the N-terminal ATPase domain based on mutants observed in primary tumors and cancer cell lines. Heterozygous expression of ATPase mutants leads to increased occupancy of Polycomb Repressive Complex 1 (PRC1) at bivalent CpG-island promoters. Increased PRC1 binding was accompanied by increases in H3K27me3, the mark left by the Polycomb Repressive Complex 2 (PRC2) ~2 kbp away.

ORGANISM(S): Mus musculus

PROVIDER: GSE77093 | GEO | 2016/10/21

SECONDARY ACCESSION(S): PRJNA309425

REPOSITORIES: GEO

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