Differential Gene Expression Time Course of Normal Human Immortalized (iPrECs) vs Tumorigenic (EMP-iPrECs) Prostate Epithelial Cells during In Vitro Prostate Epithelial Cell Differentiation

ABSTRACT: The first goal of this project was to identify differentially expressed genes associated with human prostate epithelial cells (PrEC) differentiation over a 2+ week time course that are associated with and potentially required for normal PrEC differentiation (J Cell Sci 123:266-76, 2010). The second goal was to determine how these differentially expressed genes are changed when the same cells are transformed into tumorigenic cells (EMP-iPrECs) through overexpression of Erg, Myc, and shRNA to Pten (Cancer Res 74:3357-68, 2014). In the iPrECs, we readily identified many genes that were up and down regulated over time during differentiation. Genes that decreased include many cell cycle genes, and genes that increased include known differentiation markers. The EMP cells did not differentiate as previously reported and was supported by the general lack of any major changes in gene expression over the EMP time course. When iPrECs were compared to EMPs, there were over 2000 differentially expressed genes that did not overlap with the differentially expressed genes identified during the normal differentiation time course. Thus, oncogenic transformation of PrECs results in major gene expression changes and abrogates the differentiation program.

ORGANISM(S): Homo sapiens

PROVIDER: GSE77460 | GEO | 2016/08/15

SECONDARY ACCESSION(S): PRJNA310418

REPOSITORIES: GEO