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MNase-seq of Tetrahymena thermophial macronucleus (MAC) and micronucleus (MIC)

ABSTRACT: In this study, we have employed MNase-Seq to reveal distinct nucleosome distribution patterns in morphologically and functionally differentiated MIC and MAC from asexually dividing Tetrahymena cells.

ORGANISM(S): Tetrahymena thermophila CU428

PROVIDER: GSE77660 | GEO | 2016/09/14

SECONDARY ACCESSION(S): PRJNA312451

REPOSITORIES: GEO

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Tetrahymena thermophila macronucleus chromosomes originate from chromatin breakage sequence defined TAD-like structures in micronucleus chromosomes

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Distinct 3D Genome Organization in the Two Nuclei of Tetrahymena thermophila

Project description:Somatic macronucleus (MAC) and germline micronucleus (MIC) of Tetrahymena thermophila are different in chromosome numbers, sizes, functions and cohesin complex locations. Loss of cohesin complex resulted in genome-wide disappearance of topologically associating domains (TADs) and chromatin loops in mammalian cells. However, the higher-level chromatin organization in Tetrahymena thermophila which contains both cohesin free MAC and cohesin located MIC are largely unknown. Here, using the Hi-C and HiChIP methods, we reveal that, these two nuclei possess distinct three-dimensional genome structures. In the MAC, each chromosome occupies its own territory and there are no chromatin compartmentalization or chromatin domains. The chromatin loops in MAC are mainly related to chromatin structures rather than transcriptional regulation. The MIC also without chromatin compartmentalization, but with chromatin domains and the domain boundaries are consistent with chromatin breakage sites (CBSs) which indicates that each MIC chromatin domain developed to one MAC chromosome during conjugation. Besides, we found the MIC exhibits unique intra-arm and inter-chromosome interactions at the crescent stage of conjugation, when the MIC undergoes meiotic recombination.

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Cyclin Cyc2 is required for elongation of meiotic micronucleus in Tetrahymena thermophila

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