Transcriptomics

Dataset Information

0

Knockout of Pseudouridine synthase 1 (PUS1) in mice. [Brain]


ABSTRACT: In an effort to produce a mouse model of Mitochondrial Myopathy with Lactic acidosis and Sideroblastic Anemia (MLASA), we knocked out the gene for Pseudouridine synthase 1 (PUS1), an enzyme that modifies uridine to pseudouridine in many cytoplasmic and mitochondrial tRNAs, as well as other cellular RNAs. The Pus1-/- mice are viable, are born at the expected Mendelian frequency, and are non-dysmorphic. The PUS1 mRNA and certain pseudouridine modifications are absent in cytoplasmic and mitochondrial tRNAs in the Pus1-/- mice. The Pus1-/- mice display reduce exercise capacity at 14 weeks, with alterations in muscle morphology, histology, and physiology. Red gastrocnemius muscle from Pus1-/- mice shows reduced number and size of mitochondria and reduced Cytochrome C oxidase activity.

ORGANISM(S): Mus musculus

PROVIDER: GSE77817 | GEO | 2016/05/16

SECONDARY ACCESSION(S): PRJNA311639

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-05-16 | E-GEOD-77819 | biostudies-arrayexpress
2016-05-16 | E-GEOD-77820 | biostudies-arrayexpress
2016-05-16 | E-GEOD-77818 | biostudies-arrayexpress
2016-05-16 | E-GEOD-77817 | biostudies-arrayexpress
2016-05-16 | E-GEOD-77821 | biostudies-arrayexpress
2016-05-16 | E-GEOD-77822 | biostudies-arrayexpress
2016-05-16 | GSE77820 | GEO
2016-05-16 | GSE77818 | GEO
2016-05-16 | GSE77819 | GEO
2016-05-16 | GSE77822 | GEO