Transcriptome analysis of aging mouse meibomian glands
Ontology highlight
ABSTRACT: RNA-sequencing of young and old mouse eyelids identified a number of signaling pathways, including FGF and Wnt that were altered in meibomian glands and conjunctiva of aging mice.
Project description:The objective of this study is to identify human meibomian gland genes that may promote the development and/or progression of meibomian gland dysfunction. Total RNA was isolated from normals (n=6) and patients with meibomian disease (n=6). The RNA was then used in conjuction with HumanHT-12 v3 Expression BeadChips to assay differential gene expression between normal and diseased meibomian glands.
Project description:Our objective was to determine the nature and extent of androgen regulation of gene expression in the female lacrimal, meibomian,and submandibular glands, and to explore the degree to which this control is the same as in male glands. Keywords: Hormone treatment
Project description:Eda signaling plays critical role for Meibomian gland dvelopment, however, the crosstalk of Eda with other signaling is largly unknown. By comparing expreession profilings between wild-type and Eda mutant Tabby eyelids, we identified Dkk4 and Lrp6 highly expressed during mouse Meibomian gland development.
Project description:Series includes pooled (n = 5 mice per biological replicate) samples from lacrimal, meibomian, and submandibular glands of male palcebo- and testosterone-treated BALB/c mice. All experiments were run in triplicate (pooled biological replicates) on CodeLink Mouse Uniset I Microarrays. Keywords: repeat sample
Project description:Series includes pooled (n = 5 mice per biological replicate) samples from submandibular, sublingual, parotid, lacrimal, and meibomian glands of BALB/c mice. Both male and female samples were analyzed on CodeLink Mouse Uniset I Microarrays. Keywords: repeat sample
Project description:Meibomian glands (MGs) are specialized holocrine sebaceous glands embedded in the tarsal plate of the eyelids. The cell heterogeneity of MGs is not well characterized. In this study, we performed a spatial transcriptomic analysis of murine eyelid using the 10X Visium platform.
Project description:The objective of this study is to identify human meibomian gland genes that may promote the development and/or progression of meibomian gland dysfunction.
Project description:To dissect molecular mechanisms underlying Hsd3b6−/−meibomian gland phenotypes, we performed RNA-sequencing (RNA-seq) analysis using fluorescence-activated cell sorting (FACS)-purified meibomian gland cells. RNA-seq analysis was performed using FACS-purified Itgav(+);CD45(−) meibomian gland acinar cells from wildtype and Hsd3b6−/−mice.As revealed by gene ontology (GO) enrichment analysis, cells sorted from Hsd3b6−/−mice show increased expression of inflammation-related genes. On the other hand, downregulated genes exhibit the highest enrichment score for the GO term related to “regulation of anatomical structure morphogenesis”, a signature accounting for the phenotype of the meibomian gland atrophy of Hsd3b6−/− mice.
Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in immortalized human meibomian gland epithelial cells. Analysis of regulation of immortalized human meibomian gland epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like meibomian gland cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation of numerous genes in response to dihydrotestosterone incubation of immortalized human meibomian gland epithelial cells. Total RNA was obtained from immortalized human meibomian gland epithelial cells treated for 72 hours with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in an immortalized human meibomian epithelial cell line developed in our laboratory.