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Expression profiling of grass-clump dwarf in synthetic hexaploids from triploid hybrids crossed between tetraploid wheat and Aegilops tauschii

ABSTRACT: We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in grass-clump dwarf lines, which are synthetic hexaploid lines from triploid hybrids crossed between tetraploid wheat (Triticum turgidum ssp. durum cv. Langdon or T. turgidum ssp. carthlicum) and diploid wheat progenitor Aegilops tauschii (KU2025). No up-regulation of defense-related genes was observed under the normal temperature, and down-regulation of wheat APETALA1-like MADS-box genes, considered to act as flowering promoters, was found in the grass-clump dwarf lines. Together with small RNA sequencing analysis of the grass-clump dwarf line, unusual expression of the miR156/SPLs module could explain the grass-clump dwarf phenotype.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE78784 | GEO | 2016/03/02

SECONDARY ACCESSION(S): PRJNA313524

REPOSITORIES: GEO

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Expression profiling of grass-clump dwarf in synthetic hexaploids from triploid hybrids crossed between tetraploid wheat and Aegilops tauschii

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in grass-clump dwarf lines, which are synthetic hexaploid lines from triploid hybrids crossed between tetraploid wheat (Triticum turgidum ssp. durum cv. Langdon or T. turgidum ssp. carthlicum) and diploid wheat progenitor Aegilops tauschii (KU2025). No up-regulation of defense-related genes was observed under the normal temperature, and down-regulation of wheat APETALA1-like MADS-box genes, considered to act as flowering promoters, was found in the grass-clump dwarf lines. Together with small RNA sequencing analysis of the grass-clump dwarf line, unusual expression of the miR156/SPLs module could explain the grass-clump dwarf phenotype. Expression patterns were compared between the three synthetic hexaploid lines showing the wild-type phenotype (as a reference) and grass-clump dwarf. Total RNA samples were isolated from crown tissues of the plants grown at 24Â°C under long day (18-h light and 6-h dark) condition for 8 weeks. Two independent experiments were conducted in each exprement.

2016-03-02 | E-GEOD-78784 | biostudies-arrayexpress

Expression profiling of grass-clump dwarf in syntheitic hexaploids from triploid hybrids crossed between tetraploid wheat and Aegilops umbellulata.

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in a grass-clump dwarf line, which is a synthetic hexaploid line from triploid hybrids crossed between tetraploid wheat (Triticum turgidum ssp. durum cv. Langdon) and a diploid wheat relative Aegilops umbellulata (KU-4052). Up-regulation of metabolic and catabolic processes-related genes for cell wall-associated molecules was observed, and down-regulation of wheat APETALA1-like MADS-box genes, considered to act as flowering promoters, was found in the grass-clump dwarf line. Unusual expression of the branching-related SPLs and flowering time regulation-related MADS-box genes could explain the grass-clump dwarf phenotype.

2017-01-24 | GSE93969 | GEO

Accelerated senescence and enhanced disease resistance in hybrid chlorosis lines derived from interspecific crosses between tetraploid wheat and Aegilops tauschii

Project description:Hybrid chlorosis, one of the hybrid incompatibilities, has frequently been reported in inter- and intraspecific crosses of allopolyploid wheat. In our previous study, hybrid chlorosis was observed in the wheat triploids between a tetraploid wheat cultivar Langdon and four Ae. tauschii accessions and in their derived synthetic hexaploids. However, the molecular mechanisms underlying hybrid chlorosis are not well understood. Here, we performed cytological and comparative gene expression analyses in leaves to characterize the abnormal growth in wheat synthetics showing mild and severe chlorosis symptom. In addition, disease resistance was comparatively assessed. A number of carbohydrate metabolism- and defense-related genes were markedly up-regulated in the hybrid chlorosis lines, and abnormal chloroplasts were formed in the mesophyll cells before the leaves turned to be yellowish. The mild chlorosis plants showed increased resistance to a wheat blast fungus, although little significant differences of agricultural traits were found between the wild-type and mild chlorosis-showing plants. These observations suggest that the senescence processes might be accelerated in the hybrid chlorosis lines of wheat synthetics. Moreover, the negative effects on biomass can be minimized and the substantial fitness may be obtained under pathogen-polluted conditions in the mild chlorosis-showing wheat synthetics.

2014-08-12 | GSE59640 | GEO

Expression profiling of cold-induced hybrid necrosis in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii.

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in cold-induced hybrid necrosis lines, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found in the leaves and shoot apical meristem (SAM) of type II necrosis line with low temperature. On the other hand, cell cycle-related genes were highly down-regulated in SAM of type II necrosis line under low temperature. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 37selected genes were performed in total. Of the examined 21 up-regulated and 16 down-regulated genes, more than 75% of expression pattern were consistent with the microarray data. Together with cytological analysis of the necrotic tissues, the microarray analysis strongly suggests that an autoimmune response-like reaction and repression of cell division might be triggered by intergenomic incompatibility between the AB and D genomes in type II necrosis.

2010-10-07 | GSE24566 | GEO

Accelerated senescence and enhanced disease resistance in hybrid chlorosis lines derived from interspecific crosses between tetraploid wheat and Aegilops tauschii

2014-08-12 | E-GEOD-59640 | biostudies-arrayexpress

Expression profiling of hybrid necrosis in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in hybrid necrosis-showing plants, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found, whereas photosythesis-related genes down-regulated in the type I necrosis line. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 22 selected genes were performed. Of the examined 15 up-regulated and 7 down-regulated genes, the expression pattern of only one down-regulated gene was inconsistent with the microarray data. Together with cytological analysis of the necrotic tissues, the microarray analysis strongly suggests that an autoimmune response might be triggered by intergenomic incompatibility between the AB and D genomes in type I necrosis, and that genetically programmed cell death could be regarded as a hypersensitive response-like cell death similar to that observed in Arabidopsis intraspecific and Nicotiana interspecific hybrids.

2009-12-23 | GSE19613 | GEO

Expression profiling of hybrid necrosis in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in hybrid necrosis-showing plants, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found, whereas photosythesis-related genes down-regulated in the type I necrosis line. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 22 selected genes were performed. Of the examined 15 up-regulated and 7 down-regulated genes, the expression pattern of only one down-regulated gene was inconsistent with the microarray data. Together with cytological analysis of the necrotic tissues, the microarray analysis strongly suggests that an autoimmune response might be triggered by intergenomic incompatibility between the AB and D genomes in type I necrosis, and that genetically programmed cell death could be regarded as a hypersensitive response-like cell death similar to that observed in Arabidopsis intraspecific and Nicotiana interspecific hybrids. Expression patterns were compared between the two synthetic hexaploid lines showing the wild-type phenotype (as a reference) and hybrid necrosis. Total RNA samples were isolated from the 3-week-old seedling leaves. Two independent experiments were conducted.

2010-05-16 | E-GEOD-19613 | biostudies-arrayexpress

Expression profiling of cold-induced hybrid necrosis in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii.

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in cold-induced hybrid necrosis lines, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found in the leaves and shoot apical meristem (SAM) of type II necrosis line with low temperature. On the other hand, cell cycle-related genes were highly down-regulated in SAM of type II necrosis line under low temperature. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 37selected genes were performed in total. Of the examined 21 up-regulated and 16 down-regulated genes, more than 75% of expression pattern were consistent with the microarray data. Together with cytological analysis of the necrotic tissues, the microarray analysis strongly suggests that an autoimmune response-like reaction and repression of cell division might be triggered by intergenomic incompatibility between the AB and D genomes in type II necrosis. Expression patterns were compared between the two synthetic hexaploid lines showing the wild-type phenotype (as a reference) and cold-induced hybrid necrosis. Total RNA samples were isolated from the 3-week-old seedling leaves. In addition, total RNA were also extracted from the seedling leaves and shoot meristem with 12- and 6-weeks of low temperature, respectively. Two independent experiments were conducted in each exprement.

2010-10-07 | E-GEOD-24566 | biostudies-arrayexpress

Expression data from Y12-3 and A24-39 roots

Project description:Roots adaptation to drought stress was analyzed using transcriptome and metabolomics profiles in two wild emmer wheat (Triticum turgidum ssp. dicoccoides) genotypes: Y12-3 (drought resistance) and A24-39 (drought susceptible).

2013-01-01 | GSE25940 | GEO

Expression profiling of severe growth abortion (SGA) in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii.

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in SGA lines, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found in crown tissues of SGA line. On the other hand, photosynthesis-related genes were highly down-regulated in crown tissues of SGA line. In addition, transcript accumulation levels of shoot apical meristem (SGA)-related genes such as knotted-1 like homeobox (Knox) genes were also repressed in SGA line. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 23 selected genes were performed in total. Of the examined genes, 20 genes (87%) of expression pattern were consistent with the microarray data. The microarray analysis strongly suggests that an autoimmune response-like reaction might be triggered by intergenomic incompatibility between the AB and D genomes in SGA. In addition, SAM-related and cell cycle-related genes were dramatically down-regulated in crown tissues of SGA, indicating that abnormalities of SAM are associated with the abnormal growth phenotypes in SGA. Expression patterns were compared between the two triploid hybrids showing the wild-type phenotype (as a reference) and severe growth abortion. Total RNA samples were isolated from the 3-week-old crown tissues. Two independent experiments were conducted in each exprement.

2011-11-01 | E-GEOD-33357 | biostudies-arrayexpress

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