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Ultraefficient irCLIP platform for characterization of protein-RNA interactions


ABSTRACT: The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce an advanced UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, nonisotopic method for the detection of protein-RNA interactions using 100-fold less material than standard protocols.

ORGANISM(S): Homo sapiens

PROVIDER: GSE78832 | GEO | 2016/04/25

SECONDARY ACCESSION(S): PRJNA314085

REPOSITORIES: GEO

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