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Microarray analysis to unravel the function of C-terminus domain of EBF1 by gain-of function approach

ABSTRACT: To study the function of C-terminus domain of EBF1, a gain-of function microarray analysis was performed by transducing Empty, Ebf1wt and Ebf1DC expressing vectors into Ebf1-/- progenitor cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE78842 | GEO | 2016/05/09

SECONDARY ACCESSION(S): PRJNA314126

REPOSITORIES: GEO

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Genome-wide DHS study to unravel the function of EBF1 C-terminus domain

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Microarray analysis to unravel the function of C-terminus domain of EBF1 by replacement approach

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Project description:gene expression data from mouse adipocyte, with and without Ebf1 knock-down To ascertain the functional targets of Ebf1 in adipocytes, we depleted Ebf1 from mature 3T3-L1 cells by transducing them with lentivirus harboring one of two different Ebf1-specific hairpins or a scrambled control. We then performed expression profiling using Affymetrix arrays with triplicate samples for each hairpin.

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Project description:Here, we report that EBF1 and Pax5 collaborate in a dose-dependent manner to regulate the IL-7-STAT5 signaling pathway and one-carbon metabolism, whereby we found both diminished and enhanced binding of EBF1 and Pax5 to target genes in compound heterozygous mutant mice. Moreover, single-cell RNA sequencing analysis identified a small subset of wild-type pro-B cells on the trajectory to pre-B cells that share gene expression signatures with leukemic Ebf1+/−Pax5+/− pro-B cells. Thus, a normal expression level of EBF1 and Pax5 is required for safeguarding a potentially vulnerable pro-Bcell subset from a transformation to B-ALL.

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