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Mouse popliteal lymph node transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [microRNA]

ABSTRACT: The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cortex infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt).

ORGANISM(S): Mus musculus

PROVIDER: GSE78887 | GEO | 2016/03/19

SECONDARY ACCESSION(S): PRJNA314303

REPOSITORIES: GEO

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Mouse popliteal lymph node transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [mRNA]

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Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cerebellum infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt). Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 2 and 4 days post-infection. GSE77161 (WCT001) and this study (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77161 (WCT001). Infectivity (titer) and pathology are measured from cortex tissue in GSE77161 (WCT001).

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Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cortex infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt). Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 2 and 4 days post-infection. This study (WCT001) and GSE77160 (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77160 (WCB001). Infectivity (titer) and pathology are measured from the tissue in WCT001 (i.e., this experiment).

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