Transcriptomics

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Input Strategy for Improving Analysis of ChIP-exo Data and Beyond [RNA-Seq]


ABSTRACT: Several recently emerging ChIP-seq (chromatin immunoprecipitation followed by sequencing) based methods perform chemical steps on bead-bound immunoprecipitated chromatin, posing a challenge for generating similarly treated input controls required for bioinformatics and data quality analyses. Here we present a versatile method for producing technique-specific input controls for ChIP-based methods that utilize additional bead-bound processing steps. Application of this method allowed for discovery of a novel CTCF binding motif from ChIP-exo data.

ORGANISM(S): Homo sapiens

PROVIDER: GSE79564 | GEO | 2016/08/24

SECONDARY ACCESSION(S): PRJNA316189

REPOSITORIES: GEO

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