Transcriptomics

Dataset Information

0

Molecular profile of tumor-specific CD8+ T cell hypofunction in cancer


ABSTRACT: Comparison of genome-wide mRNA expresson between tumor-infiltrating CD8+ T cells from the tumor (hypofunctional T cells) and periphery (functional T cells) Mechanisms of self-tolerance often result in CD8+ tumor-infiltrating lymphocytes (TIL) with a hypofunctional phenotype incapable of tumor clearance. Using a solid tumor model in mice, we found that CD8+ T cells became tolerized in less than 24 hours in an established tumor environment. To define the collective impact of pathways suppressing TIL function, we compared genome-wide mRNA expression of tumor-specific CD8+ T cells from the tumor and periphery. Notably, gene expression induced during TIL hypofunction more closely resembled self-tolerance than viral-exhaustion. Differential gene expression was refined to identify a core set of genes that defined hypofunctional TIL; these data comprise the first “molecular profile” of tumor-specific TIL that are naturally responding and represent a polyclonal repertoire. The molecular profile of TIL was further dissected to determine the extent of overlap and distinction between pathways that collectively restrict T cell functions. As suggested by the molecular profile of TIL, protein expression of inhibitory receptor LAG-3 was differentially regulated throughout prolonged late-G1/early-S phase of the cell cycle. Our data may accelerate efficient identification of combination therapies to boost anti-tumor function of TIL specifically against tumor cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE79858 | GEO | 2016/04/03

SECONDARY ACCESSION(S): PRJNA317238

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-04-03 | E-GEOD-79858 | biostudies-arrayexpress
| 63320 | ecrin-mdr-crc
2017-03-01 | GSE79127 | GEO
2012-02-16 | E-GEOD-32025 | biostudies-arrayexpress
2013-01-03 | E-GEOD-43260 | biostudies-arrayexpress
2018-05-26 | GSE114944 | GEO
2020-08-10 | GSE151669 | GEO
2023-07-17 | GSE218005 | GEO
2023-07-17 | GSE218004 | GEO
2021-04-08 | GSE156342 | GEO